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頁籤選單縮合
題 名 | Using Buffy Coat for Reverse Transcriptase-polymerase Chain Reaction in the Diagnosis of Dengue Virus Infection: Preliminary Study=利用血塊黃層作反轉錄酶--聚合酶連鎖反應以診斷登革病毒之感染:初期研究 |
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作 者 | 馮長風; 李元明; 郭英調; 楊素盆; 詹宇鈞; 劉正義; 何橈通; 劉武哲; | 書刊名 | 微免與感染雜誌 |
卷 期 | 33:4 2000.12[民89.12] |
頁 次 | 頁217-222 |
分類號 | 415.239 |
關鍵詞 | 反轉錄酶--聚合酶連鎖反應; 登革病毒; Buffy coat; Dengue infection; Reverse transcriptase-polymerase chain reaction; RT-PCR; |
語 文 | 英文(English) |
英文摘要 | Using reverse transcriptase-polymerase chain reaction (RT-PCR) to detect and type from viremic human serum samples for dengue virus infection is widely used today. However, a few false-negative results were reported due to very low titers of the virus particle in serum samples. As mononuclear cells, macrophages or monocytes are target cells for dengue virus infection, and the replication of virions can be observed in peripheral leukocytes frequently, the amount of virus particle in buffy coat should be higher than those in serum samples. Here, we describe a procedure in which RNA extraction from the buffy coat of a patient with a false-negative serum sample yielded specific viral RNA amplifiable by RT-PCR, thereby providing an alternative choice for the accurate diagnosis of dengue infection. |
本系統中英文摘要資訊取自各篇刊載內容。