頁籤選單縮合
題名 | Recovery of Acinetobacter Radioresistens Lipase by Hydrophobic Adsorption on a Nonwoven Fabric=以不織布行親油性吸附回收產自Acinetobacter radioresistens的脂肪酵素 |
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作者 | 陳明怡; 王惠民; 陳志勇; 陳特良; 吳昭燕; Chen, Ming-i; Wang, Hui-min; Chen, Chuh-yung; Chen, Teh-liang; Wu, Jau-yann; |
期刊 | Journal of the Chinese Institute of Chemical Engineers |
出版日期 | 20001100 |
卷期 | 31:6 2000.11[民89.11] |
頁次 | 頁595-599 |
分類號 | 460.025 |
語文 | eng |
關鍵詞 | 不織布; 親油性吸附; 回收; 脂肪酵素; Lipase recovery; Acinetobacter radioresistens; Hydrophobic adsorption; Nonwoven fabric; |
中文摘要 | 本文探討以不織布行親油性吸附回收醱液中產自Acinetobacter radioresistens的脂肪酵素;此不織布纖維之核心為聚丙烯,表面則包覆聚乙烯。脂肪酵素在不織布和Amberlite樹脂(XAD-4, XAD-16, XAD-1180, XAD-7HP)ㄎ的吸附平衡關係皆遵循Freudlich模式,其中,脂肪酵素的吸附量是以酵素活性表示之。和Amberlite樹脂相較,不織布對脂肪酵素的吸附能力最大。脂肪酵素在不織布上的吸附平衡式可用以準確地預測二階段橫向吸附之操作。從不織布脫附脂肪酵素,以0.05 M的尿素溶液效果最好;一階段脫附的回收率為55%,假如說附操作兩次,則回收率為85%。將不織布填充在20公分的管柱中,吸附脂肪酵素至飽和,再以0.05 M的尿素溶液脫附之,回收率可達89%。不織布的價格低廉且容易取得,因此有很大的增力可被應用在分離純化的操作中。 |
英文摘要 | The lipase of Acinetobacter radioresistens was recovered from fermentation broth by adsorption on a hydrophobic nonwoven fabric, which consists of a polypropylene core and a polyethylene surface. The adsorption isotherm of the lipase on the nonwoven fabric was compared with that of Amberlite resins (XAD-4, XAD-16, XAD-1180, XAD-7HP). It was found that the Freudlich model, expressed on the basis of lipase activity, could describe all of the adsorption isotherms and the nonwoven fabric possessed the highest binding capacity for the lipase. The adsorption isotherm on the nonwoven fabric could precisely predict the operation of a two-stage crosscurrent adsorption for desorption of lipase from the nonwoven fabric, 0.05 M urea was found to the most effective among those examined. For a single-state desorption, the recovery yield (based on the adsorbed lipase observed was 55%, and could reach 85% if the desorption were performed twice. Applying the nonwoven fabric in a 20-cm adsorption column and eluted with 0.05 M urea, lipase recovery, based on that had been adsorbed, could reach 89%. Taking a further count of its low price and availability, the nonwoven fabric could have a great potential being used in the downstream processing. |
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