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題名 | 黃連素合併放射線對肉瘤180細胞的研究=Effect of Berberine Combined with Argon Ion Laser and Gamma Ray on Sarcoma 180 Cells of Mice |
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作者姓名(中文) | 黃蕙棻; 游智勝; 郝道猛; | 書刊名 | 中國醫藥科學雜誌 |
卷期 | 1:3 2000.09[民89.09] |
頁次 | 頁147-155 |
分類號 | 414.51 |
關鍵詞 | 黃連素; 氬離子雷射; 伽傌射線; 肉瘤180細胞; Photodynamic therapy; Photosensitizer; Berberine; Laser irradiation; Radiation sarcoma-180 cell; |
語文 | 中文(Chinese) |
中文摘要 | 本研究的目的在於探討黃連素單獨或合併使用氬離子雷射與伽傌射線 對肉瘤180細胞的毒殺效應。將肉瘤180細胞以不同濃度的黃連素處理數小時 後,以MTT分析法來計算其存活率。另合併雷射與伽傌射線,以觀察其殺滅效 應。實驗至少重複三次。為探討黃連素合併雷射或伽傌射線對肉瘤180細胞形態 改變的改變的影響,經病理處置後,在光學顯微鏡下觀察細胞形態的改變。結果 顯示黃連素對肉瘤180細胞的半致死濃度為135μg/ml。在合併低能量雷射處理 後,當雷射能量大於12J/cm2時,其半致死濃度下降到20μg/ml以下。合併輻射 處理後,當輻射劑量為3Gy時,其半致死濃度下降到40μg/ml以下。經過黃連素 處理的肉瘤180細胞在細胞形態上的變化,包括細胞崩解、空泡形成、以及退化 性變化。 |
英文摘要 | The cell-killing effect and its possible mechanism of berberine used alone or in combination with agron ion laser and irradiation Sarcoma-180 cells were studied. The survival fraction of sarcoma-180 cells after single treatment of berberine has been investigated by MTT assay. The LC50 (half lethal concentration) of berberine has been estimated to be 135μg/ml. After the addition of low energy laser treatment (12J/cm2) the LC50 of berberine markedly decreased to 20μ g/ml. And after the addition of radiation treatment (3Gy), the LC50 of berberine decreased to 40μ g/ml. Moreover when morphologic change was examined, the sarcoma-180 cells showed cytolysis, vacuolization and degeneration after berberine treatment. The result reveals that the cell-killing effect of berberine combined with laser or radiation treatment on sarcoma-180 cells, hence introducing the possibility of berberine as a photosensitive and radiosensitive agent. |
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