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題 名 | Morphological Changes and Competence of Maturing Oocytes in the Protandrous Black Porgy, Acanthopagrus Schlegeli=黑鯛卵細胞發育及成熟之形態與反應特性 |
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作 者 | 岳文勛; 張清風; | 書刊名 | 動物研究學刊 |
卷 期 | 39:2 2000.04[民89.04] |
頁 次 | 頁114-122 |
分類號 | 388.5 |
關鍵詞 | 黑鯛; 促進成熟之類固醇; 卵細胞培養; 卵細胞成長; 卵細胞成熟; Black porgy; Maturation-inducing steroid; Oocyte culture; Oocyte growth; Oocyte maturation; |
語 文 | 英文(English) |
中文摘要 | 本研究之目的在探討黑鯛卵細胞發育與成熟之形態特徵及對促進成熟之類固醇(maturation-inducing steroid)之反應性。卵細胞發育可分為卵黃堆積前之成長期、卵黃堆積期、最後成熟與排卵,卵徑可由不到0.1mm(一年齡與二年齡)發育至接近1mm。在卵黃堆積期之黑鯛卵巢所含有之卵徑均勻分布於0-400μm之間,而剛進入成熟期之卵細胞卵徑主要是分布在350-500μm,另外有少部分卵的卵徑大於600μm。黑鯛在經過促性腺激素釋放素刺激後,約20-28小時可達卵細胞成熟,36-60小時可達排卵階段。以卵細胞培養條件下,約需4-8小時可完成卵細胞成熟過程,約需40分鐘完成排卵過程。兩種類固醇激素(17,20β-dihydroxy-4-pregnen-3-one與17,20β,21-trihydroxy-4-pregnen-3-one)皆可促進黑鯛卵細胞成熟。依卵細胞對繳素的反應特性,可將黑鯛卵細胞成熟期區分為“對激素不感應期”、“對類固醇感應期”與“卵細胞可自然成熟期”。 |
英文摘要 | The study objectives aimed to investigate the morphological changes of oocytes during final oocyte maturation and ovulation, and the maturational competence of oocytes to respond to maturation-inducing steroids in the protandrous black porgy, Acanthopagrus schlegeli. Females were injected with 2 successive doses of LHRH analog (10 and 50 μg per fish) on days 0 and 1, The oocytes were obtained at 6- or 3-h intervals from the genital pore after treatments. Morphological changes during oocyte maturation and ovulation were observed, and oocyte diameters in in vitroculture (with or without hormones) were measured. Oocyte maturation and ovulation occurred at 20-28 h and 36-60 h after the 2nd LHRH-A injection, respectively. It took 4-8 h to complete the process of oocyte maturation (invivo) and 40 min for ovulation (in vitro). Hormones (17,20β-dihydroxy-4-pregnen-3-one, 17,20β,21-trihydroxy-4-pregnen-3-one, and HCG) could significantly induce oocyte maturation in oocytes collected at 9 and 6 h prior to the occurrence of germinal vesicle breakdown (GVBD). Oocyte maturation spontaneously occurred in oocytes collected at 3 h prior to GVBD. HCG showed no synergistic effects with steroids in the induction of oocyte maturation. |
本系統中英文摘要資訊取自各篇刊載內容。