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題 名 | The Isolation and Characterization of Chaperonin 60 from Sweet Potato Roots--Involvement of the Chaperonins in Starch Biosynthesis=甘藷塊根Chaperonin 60之分離及檢定--其澱粉生合成的角色 |
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作 者 | 張世宗; 林珮君; 陳翰民; 吳建興; 莊榮輝; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 41:2 2000.04[民89.04] |
頁 次 | 頁105-111 |
分類號 | 346.78 |
關鍵詞 | 造粉體; 異常形成層; 組織定位法; 澱粉生合成; 澱粉磷解酶; Amyloplast; Anomalous cambium; Chaperonins; Histochemical methods; Ipomoea batatas; Starch biosynthesis; Starch phosphorylase; |
語 文 | 英文(English) |
中文摘要 | 由甘藷塊根中可分離得 molecular chaperonins cpn60a 及 cpn60b,並檢定為同質多元體,但兩者的免疫及生物化學性質有相當大的差異性。這兩種 cpn 在塊根充實的過程中,與澱粉磷解瓷同時出現,並以組織定位方法得知位於澱粉粒上。當甘藷開始生合成澱粉時,在塊根異常形成層 (anomalous cambium) 附近的細胞中,三種蛋白質的量都有顯著增加。以上觀察顯示, chaperonins 可能對甘藷澱粉的生合成有重要的生理功能。 |
英文摘要 | Molecular chaperonins cpn60a and cpn60b were isolated from sweet potato roots and were characterized as homologous polymers. However, their immunochemical and biochemical properties were divergent. The two chaperonins as well as starch phosphorylase appeared concurrently during the starch-accumulating period and were localized in the starch granule by histochemical methods. Notably, these proteins increased substantially in cells around the anomalous cambium when sweet potato initiated starch biosynthesis. Observations in this study indicated that the chaperonins might be involved in the biosynthesis of starch in sweet potato roots. |
本系統中英文摘要資訊取自各篇刊載內容。