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頁籤選單縮合
題名 | Expression of Rice Prolamin RP3 Promoter Exhibits a Positive Association with Cellular Pigment Contents in Transgenic Tobacco Suspension Cultures=轉殖菸草懸浮細胞內水稻醇溶蛋白RP3起動子的表現與色素含量有正相關性 |
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作 者 | 陳顯榮; 楊智元; 陳琰青; 陳慶三; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 41:2 2000.04[民89.04] |
頁次 | 頁93-98 |
分類號 | 434.823 |
關鍵詞 | 醇溶蛋白質; 水稻; RP3起動子; 色素; 菸草; Pigment; Prolamin; Rice; RP3 promoter; Tobacco; |
語文 | 英文(English) |
中文摘要 | 三個含有水稻醇溶蛋白 RP3/GUS chimeric gene inserts 之轉植菸草,植株(品系 RP3/2,RP3/7 及 RP3/18)已被分離及建立懸浮細胞培養(Yang et al., 1999),細胞品系間差異性的 GUS 表現及形態、生化、及分子的變異亦被觀察到(Chen et al., 1999)。在這篇報告中我們使用色素如類葫蘿蔔素、葉綠素 a 及 b 作為標誌探討 RP3 起動子活性與細胞色素含量的可能關聯,並且發現此二者於細胞品系間有正相關性。RP3/2 的懸浮細胞具有最多的色素含量及最大的 GUS 表現量,RP3 起動子活性在此懸浮細胞內 12 天的繼代培養過程中逐漸的減少,而且與細胞色素含量下降量平行。於 RP3/18,於細胞生長過程該二者亦顯示平行的關聯性且保持相當的持平。由這些實驗結果顯示水稻醇溶蛋白 RP3 起動子活性在轉殖菸草懸浮細胞品系間及品系內與色素含量有正相關性。我們對該關聯之意義及可能的解釋提出一些看法。 |
英文摘要 | Three transgenic tobacco regenerants (lines RP3/2, RP3/7 and RP3/18) harboring rice RP3/GUS chimeric gene inserts in their genomes were isolated previously and used to establish cell suspension cultures (Yang et al., 2000). A differential GUS expression pattern together with morphological, biochemical, and molecular variations were observed among these cell lines (Chen et al., 1999). In this report we used pigments such as carotenoids and chlorophylls a and b as markers to study the possible relationship between RP3 promoter activity and cellular pigment contents, and a positive association was found among these cell lines. RP3/2, which contained the highest cellular pigment contents, also exhibited the highest GUS expression level. The RP3 promoter activity in RP3/2 continuously decreased and was parallel with the reduction of pigment contents within a 12-day growth period after subculture. In RP3/18, both also showed a parallel association and remained relatively constant during cell growth. Based on these results, a positive association between RP3 promoter activity and cellular pigment content was found among and within cell lines. The meaning of the association and its possible explanation are discussed. |
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