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題 名 | 溶磷菌Pseudomonas cepacia A1-74菌株分解磷酸三鈣的特性研究=Studies on Properties of Solubilizing Tricalcium Phosphate of Pseudomonas cepacia Al-74 Strain |
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作 者 | 楊秋忠; 黃昭欽; 林大發; | 書刊名 | 中華農學會報 |
卷 期 | 1:2 2000.04[民89.04] |
頁 次 | 頁150-158 |
分類號 | 434.225 |
關鍵詞 | 溶磷微生物; 分子篩; 分泌物; 生物代謝產物; Pseudomonas cepacia; Phosphate-solubilizing microorganisms; Molecular sieve; Metabolite; |
語 文 | 中文(Chinese) |
中文摘要 | 溶磷微生物於分解難溶性磷酸化合物時的機制複雜,爭議甚多。本研究的目的在於探討溶鈣磷細菌Pseudomonas cepacia A1-74菌株分泌溶磷物質之分子量與培養基中水溶性磷濃度的關係。以分別測量A-74菌株生長特性曲線、孔隙阻礙溶磷現象及分子篩試驗,結果顯示A- 74菌株分解培養基中磷酸三鈣的能力與培養基中菌種分泌之有機物有相關,並證明氫離子不是A1-74菌株分解磷酸三鈣的主要物質,而在A1-74菌株上分泌物的分子量介於500至1000道耳頓(Da)的物質是分解磷酸三鈣的主要物質。A-74菌株的溶磷表現中,氫離子和其他小分子有機酸(分子量 < 500 Da)只貢獻36.2%,而分子量介於500至1000Da的物質貢獻43.5%,所以A1-74菌株分泌物中分子量介於500至1000 Da的物質是分解培養基中磷酸三鈣的主要物質,值得進一步研究確切的溶磷物質,而氫離子可能是由該磷溶物質所釋放的次產物。 |
英文摘要 | The mechanisms of solubilizing phosphate of Phosohate-solubilizing microorganisms are complex, such as the effect of organic acids, pH, NH₄+ assimilation and respiration, especially the role of H+ and the pH variability. Therefore, we focused on the growth properties of Pseudomonas cepacia A1-74 strain, and effects of pore size and molecular sieve to realize the relationship of metabolites of phosphate-solubilizing microorganisms and the water-soluble phosphate during solubiling phosphate process. The result showed that the amount of water-soluble phosphate ws apparently increased during log phase, and the pH was decreased at the same time. Meanwhile, pH was increased at mid and last stationary phase but the amount of water-soluble phosphate was gradually decreased. In addition, the result of molecular sieve experimene showed that the molecular weight between 500 and 1000 Da of metabolites of Pseudomonas cepacia A1-74 strain played an essential role on solubilizing phosphate since 43.5% of water-soluble phosphate was detected and 36.2% was contributed by low molecular metabolites ( < 500 Da). These results showed that metabolites (500-1000 Da) of Pseudomonas cepacia A1-74 strain played more essential role than metabolites ( < 500 Da) and pH effect on solubilizing phosphate. |
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