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頁籤選單縮合
題名 | Phosphate Starvation Induction of Acid Phosphatase in Ulva Lactuca L. (Ulvales, Chlorophyta)=缺磷誘導石蓴(Ulva lactuca L.)酸性磷酸分解酵素活性增高 |
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作者 | 李澤民; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 41:1 2000.01[民89.01] |
頁次 | 頁19-25 |
分類號 | 379.27 |
關鍵詞 | 酸性磷酸分解酵素; 鹼性磷酸分解酵素; 缺磷; 石蓴; Acid phosphatase; Algae; Alkaline phosphatase; Phosphate starvation; Ulva lactuca; |
語文 | 英文(English) |
中文摘要 | 本研究探討大型海洋綠藻石蓴( Ulva lactuca L. )經 4 天的 1、15 或 30μM Nah �� PO �眾B理後,比生長速率、細胞內無機磷酸含量、總磷含量、酸性磷酸分解酵素( ACP;EC 3.1.3.2 )比活性及鹼性磷酸分解酵素( AP;EC 3.1.3.1 )比活性之關係。比生 長速率、細胞內無機磷酸含量及總磷含量隨 NaH �� PO �窒@度增高而增加。 比生長速率與 細胞內無機磷酸含量成直線相關( r �� =0.78,P<0.05 ),與總磷含量也成直線相關( r �� =0.83,P<0.05 )。暗示石蓴與磷酸的利用性有關。ACP 的比活性是 AP 比活性的 4 ∼ 10 倍。 在 1 μ M NaH �� PO �略U,增加而 AP 比活性降低。ACP 比活性與生長速率成直 線相關( r �� =0.72, P<0.05 ), 與細胞內無機磷酸含量也成直線相關 (r �� =0.80, P<0.05 )。但是,AP 比活性與比生長速率或細胞內無機磷酸含量無關。利用外加於酵素反 應液的 in vitro 方式,NaH �� PO �畢b濃度高於 20 μ M 下對 ACP 及 AP 比活性有明顯 的抑制作用。 本研究的結果指出缺磷所誘導的 ACP 活性增高可能與大型海洋綠藻石蓴在缺 磷狀況下可利用磷的含量降低有關。 |
英文摘要 | The relationships among specific growth rate, intracellular inorganic phosphate (Pi) concentrations, total phosphorus (P) concentrations and specific activities of acid phosphatase (ACP; EC 3.1.3.2) and alkaline phosphatase (AP; EC 3.1.3.1) were studied in a green marine macroalga Ulva lactuca L. after 4 days of incubation at 1, 15 or 30 μ M NaH �� PO ��. Both the specific growth rate and the intracellular Pi and total P concentrations increased with increasing NaH �� PO �� levels; a liner correlation with specific growth rate was found for both intracellular Pi concentrations (r �� =0.78, P<0.05) and the total P concentrations (r �� =0.83, P<0.05). The growth rate and the Pi and total P concentrations were relatively low at 1 μ M NaH �� PO ��, indicating U. lactuca L. could be P-deficient at 1 μ M NaH �� PO ��. The specific activity of ACP was 4 to 10-fold greater than that of AP. Exposure to 1 μ M NaH �� PO �� increased the specific activity of ACP but slightly decreased that of AP. The ACP specific activity was inversely correlated with specific growth rate (r �� =0.72, P<0.05) and also intracellular Pi concentrations (r �� =0.80, P<0.05) and the total P concentrations (r �� =0.64, P<0.05). The AP specific activity had no correlation with specific growth rate, intracellular Pi concentratins, or total P concentrations. The addition of NaH �� PO �� concentrations higher than 20 μ M to the assay mixture inhibited the specific activities of both ACP and AP. Overall, the P deficiency induction of ACP activity is related to a decrease in P availability in U. lactuca L. |
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