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題 名 | Interleukin-8 Secretion by Cultured Oral Epidermoid Carcinoma Cells Induced with Nicotine and/or Arecoline Treatments=尼古丁和檳榔鹼單獨或共同處理會促使培養之口腔上皮癌細胞分泌白血球間質-八 |
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作 者 | 鄭雅愛; 薛良芬; 余幸司; 謝天渝; 蔡吉政; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 16:3 2000.03[民89.03] |
頁 次 | 頁126-133 |
分類號 | 416.9 |
關鍵詞 | 尼古丁; 檳榔鹼; 口腔; 上皮癌細胞; 白血球間質-八; Nicotine; Arecoline; IL-8; |
語 文 | 英文(English) |
中文摘要 | 白血球間質-八(interleukin-8, IL-8)是一個多功能的細胞激素。它可以在 免疫及發炎活動上扮演角色;其他如體外觀察顯示IL-8對角質細胞中為生長因 子。本研究以1至100 μM的nicotine與arecoline,單獨或是共同處理培養的口 腔癌KB CCL17細胞72小時,再以酵素連結免疫反應分析(enzyme-linked immunosorbent assay, ELISA)來測定細胞上清液中IL-8的濃度。重覆測定分析變異 數來檢測各個實驗組間的差異性。在nicotine和arecoline的共同處理的實驗中, IL-8的濃度被發現會有比對照組戲劇性增加很多。濃度1 μM的nicotine和1 μ M的arecoline共同處理會使IL-8濃度比對照組增加66.7 %; 100 μM的nicotine 和100 μM的arecoline共同處理會使IL-8濃度比對照組增加2.5倍。當外加1 μ g/ml的單一純化的IL-1α或IL-1β抗體在各個處理中,IL-8濃度會有顯著性減 少。在細胞增殖方面,IL-1α或IL-1β抗體對於對照組並沒有顯著性影響,但是 若把IL-1α或IL-1β抗體加入nicotine和arecoline的處理中,則其細胞增殖會比 未加入抗體者顯著地增加很多。這些結果建議在活體內IL-8的分泌會受到IL-1 局部產生的特殊調節。而且nicotine和arecoline會促使IL-1分泌去抑制KB CCL17 細胞的生長。因此,在吸煙和嚼檳榔所導致之一連串相關細胞激素分泌反應的發 炎或致癌作用中,IL-8可能扮演一個重要角色。 |
英文摘要 | Interleukin-8 (IL-8) is one of the multifunctional cytokines that can play a role on immune and inflammatory activities. Other in vitro observations indicated that IL-8 is a growth factor for keratinocytes. However, as the role of IL-8 in oral cancer cells is unclear, this study is thus designed to examine IL-8 secretion in cultured oral epidermoid carcinoma KB CCL17 cells treated with nicotine and/or arecoline. The cultures were treated with nicotine (1 or 100 μM) and arecoline (1 or 100 μM), alone or both, for 72 hrs. Enzyme-linked immunosorbent assay (ELISA) was used to examine IL-8 concentrations in culture supernatants. A repeated measure analysis of variance was used to identify differences among the treatments. Nicotine and arecoline, single or combined treatment, increased IL-8 secretion in KB CCL17 cells. When monoclonal 1μg/ml of antibody was added against IL-1α or IL-1β in the treatment, IL-8 concentration significantly decreased compared with the non-added one. Exposure of cells to antibody against IL-1α or IL-1β showed no significant increase in cell growth as compared with the control (medium alone). However, incubation of cells for 72 hrs in the presence of nicotine and/or arecoline treatments and antibody against IL-1α or IL-1β significantly increased cell growth as compared with the antibody free one. It was concluded that IL-8 secretion by KB CCL17 cells may be partially mediated by IL-1 which could inhibit the KB CCL17 cell growth. Thus, IL-8 may be a vital participant in the cascade of interacting cytokines during smoking and areca quid chewing, inducing inflammation in oral cancer. |
本系統中英文摘要資訊取自各篇刊載內容。