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題 名 | 應用白雲杉雌配子體「Picea Glauca (Moench) Voss Megagametophyte」組織培養研究色素形成因子=Application of Picea Glauca (Moench) Voss Megagametophyte Tissue Culture in Study of Factors Affecting Pigmentation |
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作 者 | 朱紀實; | 書刊名 | 國立臺灣大學農學院實驗林研究報告 |
卷 期 | 12:3=221 1998.09[民87.09] |
頁 次 | 頁203-211 |
分類號 | 436.259 |
關鍵詞 | 白雲杉; 雌配子體; 組織培養; 癒合組織; 植物生長調節劑; White spruce; Megagametophyte; Tissue culture; Callus; Plant growth regulator; |
語 文 | 中文(Chinese) |
中文摘要 | 針葉樹種子之雌配子體為單倍體細胞(haploid),可經組織培養方法成雙倍體細胞, 以作林木改良之用。從貯存六年之白雲杉種子,經解剖取出雌配子體並培養在MS培養基,附加 2%蔗糖、1%洋菜膠、10μM 6-benzyl-aminopurine (BAP)和10μM 2,4-dichloro-phenoxyacetic acid (2,4-D),在黑暗中,培養3-4週後,生成白色雌配子體癒 合組織。癒合組織於低(8μE/m□/sec)或高(50μE/m□/sec)光照射下,均呈白色。此癒合組 織轉植到MS培養基,含1μM Zeatin或0.5μM Zeatin和0.05μM naphthalene acetic acid(NAA), 可生成綠色或綠色及紅色相間組織,此組織繼續培養在上述培養基可誘導芽的生成。 近而利用白色癒合組織研究色素形成因子。細胞生長分裂素(Cytokinin):BAP和Zeatin都會 誘導綠色、紅色及棕色形成,但兩者反應略有不同,BAP主要誘導形成綠色組織。而2,4-D則 會抑制細胞生長分裂素的促進綠色及紅色的作用。白色癒合組織對葉綠素前衍物glutamate 及5-aminolevulinic acid (ALA)的反應亦不同,glutamate主要促進棕色,而ALA或 glutamate及ALA一起則不會促進顏色的改變。在37℃ 1小時狀況下,白色癒合組織則會轉 成棕色,並抑細胞生長分裂素所誘導的綠色形成。 |
英文摘要 | Shoot regeneration of white spruce (Picea glauca) from mature megagametophyte tissue was obtained using seeds that have been stored for six years. Megagametophyte calli were induced on MS medium (pH 5.8) supplemented with 2% sucrose, 1.0% agar, 10μM 6-benzyl-aminopurine (BAP) and 10μM 2,4-dichlorophenoxyacetic acid (2,4-D) for 3-4 weeks in the dark. Calli were subcultured on the same medium in either low light (8 μE/m□/sec) or high light (50 μE/m□/sec). Megagametophyte calli remained white in color even when grown under either light intensity. When calli were transferred onto MS medium (pH 5.8) supplemented with 1 μM zeatin or 0.5 μM zeatin and 0.05 μM naphthalene acetic acid (NAA), green and red regions appeared on white calli. After subculturing on the same medium, shoots were regenerated. Those white calli, grown on medium containing 10 μM BAP and 2,4-D, were transferred to medium supplemented with cytokinin BAP or zeatin which resulted in a change of color to green, red, or brown. The effects of zeatin differed from BAP, causing the color change to green but not to red or brown. The addition of 2,4-D to the medium inhibited the color change induced by the cytokinins, especially the development of green and red regions. White callus also showed different responses to the addition of glutamate and 5-aminolevulinic acid (ALA) into the medium. Glutamate caused browning of white calli, while ALA alone or glutamate and ALA together caused no change in color. Exposure to 37℃ for an hour also changed the response of white calli to BAP and zeatin by significantly increasing browning while decreasing the development of green colored regions. |
本系統中英文摘要資訊取自各篇刊載內容。