查詢結果分析
相關文獻
- Preferential Reduction of Na狇/K狇ATPase α3 by 17β-Estradiol Influences Contraction Frequency in Rat Uteri
- 礦物類中藥材安全性制定之前導研究--市售朱砂之藥理機制研究(2)
- Effects of Methyl Mercury, Mercuric Sulfide and Cinnabar on Active Avoidance Responses, Na狇/K狇-ATPase Activities and Tissue Mercury Contents in Rats
- The Detrimental Effects of Potassium Bromate and Thioglycolate on Auditory Brainstem Response of Guinea Pigs
- 礦物性中藥安全性制定之前導研究--市售硃砂之藥理機制研究
頁籤選單縮合
題名 | Preferential Reduction of Na狇/K狇ATPase α3 by 17β-Estradiol Influences Contraction Frequency in Rat Uteri |
---|---|
作者 | Tsai,Mei-ling; Lee,Chia-ling; Tang,Ming-jer; Liu,Ming-yie; | 書刊名 | 中國生理學雜誌 |
卷期 | 43:1 2000.03[民89.03] |
頁次 | 頁1-8 |
分類號 | 364.6782 |
關鍵詞 | Na狇/K狇-ATPase; 17β-estradiol; Uterus; Contraction; Oscillations; |
語文 | 英文(English) |
英文摘要 | One β1 and two α (αl and α3) isoforms of Na⁺/K⁺-ATPase exist in rat uteri. Previous immunocytochemistry studies have suggested that the a3 isoform may be involved in calcium regulation indirectly. Estrogens are known to both modulate Na⁺/K⁺-ATPase activities in non-uterine tissues and suppress spontaneous uterine contractions in rats. Thus the purpose of this study was to examine the correlation between estrogens-modulated uterine contraction and the expression of Na⁺/K⁺-ATPase α3 isoform in rats. After 1-, 2-, and 4- day treatments with 17β.estradiol (E2, S µg/ml/kg, s.c., daily), the diameter of uterine horn was measured. The contraction force of uterine strips was measured by standard muscle bath apparatus. The protein abundance and enzyme activity of Na⁺/K⁺-ATPase in rat uteri were measured by Western blot analysis and ATPase assay, respectively. One day of E2 decreased both contraction frequency and α3-protein expression without the change in uterine diameter, enzyme activity or other isoforms. Two days of E2 reduced contraction frequency, the enzyme activity, as well as α3- and β1- protein abundance but increased αl-protein and uterine diameter. Four days of E2 elicited similar effects as two days of E2, but did not affect αl-protein abundance. In conclusion, E2 elicits differential effects on isoform expression. After 1-day treatment with 17β-estradiol, the decrease in the expression of α3 and β1 without a change in Na⁺/K⁺-ATPase activity suggests that some isoform other than β1 exist in rat uteri. The positive correlation between the reduction of α3-and the decrease of contraction frequency suggests the involvement of α3 isoform in uterine oscillation. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。