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題 名 | Detection of Mycobacterium Tuberculosis by Nested PCR and Histological Analysis in Formalin-Fixed Paraffin-Embedded Tissue=福馬林固定後石蠟包埋標本內結核桿菌的蜂巢式聚合酶連鎖反應測試與其組織學分析 |
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作 者 | 韓鴻志; 樂國柱; 何莉櫻; 謝渙發; 邱紹智; | 書刊名 | 秀傳醫學雜誌 |
卷 期 | 2:1 2000.01[民89.01] |
頁 次 | 頁17-24 |
分類號 | 415.2773 |
關鍵詞 | 結核桿菌; 蜂巢式聚合酶連鎖反應; Mycobacterium tuberculosis; Nested polymerase chain reaction; |
語 文 | 英文(English) |
中文摘要 | 結核病在臺灣是常見的疾病,以傳統acid-fast stain (AFB) 或細菌培養,不是 診斷率太低(約 15% )就是時間太長(約 2 個月),尤其在石蠟包埋肉芽腫的診斷更是困 難。我們以蜂巢式聚合�t連鎖反應( nested PCR )來偵測福馬林固定石蠟包埋肉芽切片組 織中結核桿菌之存在, 配合組織學上的表現來進行分析。 結果發現以單一步驟放大反應在 52 個病人中有 19 人( 36.5% )呈陽性反應,而在連續性第二步驟反應,陽性率可提高至 63.5% ( 33 人)。 結核桿菌蜂巢式聚合�t連鎖反應的陽性組織學型態分析,有下列 4 種 : 典型肉芽腫( 19/33 )、類肉瘤肉芽腫( 4/33 )、類淋巴上皮肉芽腫( 9/33 )、星 狀肉芽腫( 2/33 )。另外在 33 件組織中 14 件即具微小囊腫的變化。病理學上,典型性 藍格罕士巨細胞( Langhan's giant cell )占 54.5% ( 18/33 ),同時出現典型肉芽腫 和類肉瘤肉芽腫的有 2 例,類淋巴上皮肉芽腫和典型肉芽腫同時出現的有1例。 結論:1. 以蜂巢式聚合�t連鎖反應診斷結核病是一快速、敏感之方法。2. 不同型態的肉芽 腫可能是因結核病不同階段,或者不同宿主免疫力所引起。 |
英文摘要 | A Simplified protocol based on the nested polymerase chain reaction (PCR) was developed for detection of Mycobacterium tuberculosis in the formalin-fixed paraffin-embedded chronic granulomatous inflammation tissue. By DNA ampification, PCR was positive in 19 (36.5%) of the 52 patients in the one-step amplification, whereas PCR was positive in 33(63.5%) of the 52 patients following the two-step amplification (P<0.001). Histologically, there were four types of granulomas identified: (1)classical M. tuberculosis granulomas (19/33); (2) sarcoidosis-like granulomas (4/33); (3)lympho-epithelioma-like granulomas (LELG)(9/33); (4) satellite granulomas(2/33). In addition, the microabscesses were present in 14/33. The typical Langhan's giant cells were present in 54.5% cases (18/33). Interestingly, the classical M. tuberculosis granulomas and sarcoidosis-like granulomas were present simultaneously in two cases. The LELG and classical granulomas were present simultaneously in seven cases. LELG and sarcoidosis-like granulomas were present simultaneously in one case. In summary, the nested PCR is useful for rapid diagnosis of M. tuberculosis. The variety of granulomas may be due to different stages of tuberculosis and/or the difference of individual host immunity. |
本系統中英文摘要資訊取自各篇刊載內容。