頁籤選單縮合
題名 | Determination of Xanthine Bronchodilators in Adulterated Chinese Herbal Preparations by High Performance Liquid Chromatography |
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作者 | 顧祐瑞; 溫國慶; 何禮剛; 張永勳; | 書刊名 | 藥物食品檢驗局調查研究年報 |
卷期 | 17 1999.10[民88.10] |
頁次 | 頁260-273 |
分類號 | 414.5 |
關鍵詞 | HPLC; Theophylline; Diprophylline; Traditional Chinese medicine; Adulterant; |
語文 | 英文(English) |
英文摘要 | Five xanthine bronchodilators, caffeine, diprophylline, proxyphylline, theobromine and theophylline, were simultaneously assayed in traditional Chinese medicine by high performance liquid chromatography on a Merck RP-select B column. The chromatographic method was carried out using isocratic elution with methanoi and 1% acetic acid (15:85, v/v). 8-Chlorotheophylline was used as an internal standard and detection was at 270 nm. Calibration curves of the five adulterants were constructed in the range 8-160 ug/mL for theobromine and 10- 200 ug/mL for the others. Their correlation coefficients ranged from 0.99993 to 0.99997. Extracts were treated by Supelclean C-18 before HPLC analysis. The recoveries of the adulterants in three traditional Chinese medicinal prescriptions were also studied. Diprophylline and/or theophylline were found and assayed from traditional Chinese medicines by combined solid-phase extraction and HPLC. |
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