頁籤選單縮合
題 名 | Improved Synthetic Yield of a Cationic Derivative of Cholesterol |
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作 者 | 余秀瑛; 劉乃菁; 程正禹; | 書刊名 | 中華藥學雜誌 |
卷 期 | 51:3 1999.06[民88.06] |
頁 次 | 頁241-244 |
分類號 | 418.4 |
關鍵詞 | DC-chol; Cationic cholesterol derivative; Cationic liposomes; |
語 文 | 英文(English) |
英文摘要 | A cationic derivative of cholesterol, 3β [N-(N',N'-dimethylaminoethane)-car-bamoyl] cholesterol (DC-Chol), is used as a component of cationic liposomes for efficient DNA transfection. The synthesis of DC-Chol has been reported. A modification to improve the yield of DC-Chol is described. Dry methylene chloride instead of dry chloroform was used to dissolve cholesteryl chloroformate and N,N-dimethylethylenediamine (DMEDA). Cholesteryl chloroformate solution was added dropwise to DMEDA solution at 0℃. After reaction, the solvent was evaporated and the residue was recrystallized in absolute ethanol at -20℃. The final product was identified by TLC (single spot), melting point (107.5 ~ 108.5 ℃),□H NMR spectrum and elemental analysis. The yield of DC-Chol was 73.2%, much higher than previously reported (21.8 %). The modified method offers a productive yield of DC-Chol for use in cationic liposomes. |
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