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題 名 | Dynamic Measurement of Metabolic Function in the Rat Liver: An Application of Reverse Microdialysis=逆向微透析應用於大鼠肝臟代謝功能之動態性監測 |
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作 者 | 石明煌; 陳漢明; 呂保平; 譚培炯; | 書刊名 | 長庚醫學 |
卷 期 | 22:2 1999.06[民88.06] |
頁 次 | 頁246-252 |
分類號 | 415.12 |
關鍵詞 | 逆向微透析; 利多卡因; P450細胞色素; Microdialysis; Lidocaine; Monoethylglycinexylidide; Cytochrome P450; |
語 文 | 英文(English) |
中文摘要 | 背景:傳統上,監測肝臟代謝與微粒體功能需要定時抽血及採檢。微透析技術是 一項新的採樣方法,不需要真的將組織切片或體液抽出,這項特性對小動物研究非常重要。 本研究是使用逆向微透析技術,探討其應用於大鼠肝臟代謝功能之動態性監測之可行性。方 法: 實驗動物為成熟、 雄性 Sprague-Dawley 大鼠, 以巴比妥鹽鈉 (pentobarbital sodium) 麻醉,將微透析管植入其肝臟中葉,以 lidocaine 灌流, 進行逆向微透析技術, 測量其透析液中利多卡因 (lidocaine) 及主要代謝產物 monoethylglycinexylidide (簡稱 MEGX) 濃度。 我們定義其代謝能力為 20 分鐘之中,MEGX 產量與利多卡因供應量之比值。 我們並以肝臟缺血再灌流與肝硬化兩種實驗模式驗證,分別分析比較其代謝能力動態與靜態 性改變。 結果: 植入微透析管與肝臟組織可以穩定接觸。 正常肝臟中, 估計有 4.73 ± 0.41 %利多卡因轉化為 MEGX。而硬化肝只有其 16 %代謝能力。 20 分鐘肝臟缺血引起肝 臟代謝功能減少,其再灌流後只有短暫對肝臟代謝能力有影響。結論:本研究確定植入微透 析管可以與肝臟組織穩定接觸,因此化學物質可以在微透析管內外自由且穩定進出,此特性 使逆向微透析技術可以作為評估肝臟動態性代謝功能之有用工具。 |
英文摘要 | Background:Traditional markers of liver function and microsomal activity tests require regular samplings and tissue removal. The microdialysis technique is a promising tool for pharmacokinetic study without the need for actually removing samples of those tissues and fluids. We verified the possibility of using reverse microdialysis for dynamic monitoring of hepatic metabolic function. Methods:Adult male Sprague-Dawley rats were used and anesthetized using pentobarbital sodium. Reverse microdialysis was done by implanting a microdialysis probe into the middle lobe of the liver; the probe was then perfused with a lidocaine-containing solution. Concentrations of lidocaine and its major metabolite, monoethylglycinexylidide (MEGX), were measured in the dialysate. Metabolic ability was assessed by dividing the MEGX production by lidocaine administration. Hepatic ischemia-reperfusion and liver cirrhosis models were used to verify its application in dynamic measurement of live metabolic function. Results:The implantable microdialysis probe had stable contact with the liver tissue. In normal rats, 4.73 ± 0.41% of the lidocaine was transformed to MEGX in 20 min. Only 16% of this value was preserved in cirrhotic animals. Hepatic ischemia for 20 min transiently depressed the MEGX formation and did not cause further injury after reperfusion. Conclusion:We confirmed the ability of an implantable microdialysis probe to be in constant contact with the liver tissue and thus deliver a stable transmission of chemicals across a microdialysis membrane for a certain period of time. We also verified the feasibility of reverse microdialysis as a tool for the dynamic measurement of hepatic metabolic function. |
本系統中英文摘要資訊取自各篇刊載內容。