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頁籤選單縮合
題名 | Qualitative Distinction of Carboxyl Group Distributions in Pectins with Ruthenium Red=以Ruthenium read 結合法區別果膠羧基分布 |
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作者 | 侯文琪; 張為憲; 江啟銘; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 40:2 1999.04[民88.04] |
頁次 | 頁115-119 |
分類號 | 341.91 |
關鍵詞 | 區段型; 酯化度; 果膠; 隨意型; 釕紅; Blockwise; Degree of esterification; DE; Pectin; Random; Ruthenium red; |
語文 | 英文(English) |
中文摘要 | 利用主釕紅 (ruthenium red) 溶液 , 研究探討不同商品果膠酯化度 (0%,31%,63% 和93%) 的游離 梭基的結合條件 , 最適結合的條件再進一步探討自行製備不同酪化度的果膠 (0% 到80%) 。這些果膠分別利用鹼性溶液 ( 代表隨意型分布 ) 在4〔〕 進行反應或是橘皮來源的商品果膠酯晦 ( 代表區段型分布 ) 在3O〔〕 進行反應。結果顯示 , 利用鹼性溶液及橘皮來源的商品果膠酯〔〕所製備的不同酯化度果膠和其相對的 〔〕A534m數值 , 有完全不同的相關性類型 , 而 〔〕A534m數值代表真正結合在果膠梭基上的釕紅數量。鹼性溶液所製成的不同酯化度果膠和其相對的 〔〕A534m數值 , 呈現一次迴歸負相關性。 (r= -0.995), 而橘皮果膠酯胞所製備的不同酯化度果膠和其相對的 〔〕A534m數值 , 呈現二次迴歸負相關性(r=0.998) 。利用釘紅染劑進行果膠的結合 , 可以區別梭基在果膠長鏈上的分布是區段型還是隨意型。 |
英文摘要 | Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding AA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules. |
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