頁籤選單縮合
| 題 名 | 山藥種原外表形態及分子標記之變異研究=Study on Morphological and Molecular Markers of Yam (Dioscorea Spp.) Germplasm |
|---|---|
| 作 者 | 賴瑞聲; 高瑞隆; 林義恭; 胡敏夫; 劉新裕; | 書刊名 | 臺灣農業研究 |
| 卷 期 | 54:3 民94.09 |
| 頁 次 | 頁195-206 |
| 分類號 | 434.3 |
| 關鍵詞 | 山藥; 外表形態; 分子標記; Yam; Morphological trait; Molecular marker; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 三十二個山藥參試品種(系)屬於8個物種及1個變種,包括Dioscorea alata L.(17個)、D. alata L. var. purpurea(3個)、D. batatas(5個)、D. pseudojaponica(2個)、D. doryphora(1個),D. escunlenta(1個)、D. bulbifera(1個)、D. rotundata(1個)及D. persimilis(1個)進行外表形態調查及RAPD分子性狀分析,以探討物種間及物種內之變異。取其葉片DNA進行RAPD分析,在40個逢機引子對中,有12個引子對可穩定擴增出43個多型性片段,進一步進行群組分析時可明確分成七群,與物種歸屬符合,其中以D. alata為最大群,種內可分為五小群,分別為塊狀薯群、罐形薯群、紅皮白肉山藥群、紅肉山藥群、長薯山藥群,而優良品系大汕二號與台農2號明顯不同。儘管同一群內品種(系)分子鑑定仍有困難,但外表形態分群結果與RAPD分析分群相呼應,表示可依種群進行特色開發,進行群內品系選育,以達到多樣化育種目的,可讓消費者有更明確的選擇。 |
| 英文摘要 | Thirty-two yam lines of nine species, including Dioscorea alata L. (17 lines), D. alata L. var. purpurea (3 lines), D. batatas (5 lines), D. pseudojaponica (2 lines), D. doryphora (1 line), D. escunlenta (1 line), D. bulbifera (1 line), D. rotundata (1 line) and D. persimilis (1 line) were used in this study. Variation among and within yam species was investigated via morphological and RAPD analysis. Forty random primers were carried out in RAPD analysis. Forty-three polymorphic bands generated by twelve primers were used in cluster analysis. The result reveals seven clusters consistent with classification yam species. D. alata species is the largest cluster, and can be further divided into five sub-clusters. The first sub-cluster is clumpy yam. The second sub-cluster is bottle-shape yam. The third sub-cluster is white flesh with red skin yam. The forth sub-cluster is red flesh yam. Another one is long tuber length yam. Although it is still difficult to identify specific line within the same cluster, such a RAPD analysis corresponds with morphological analysis reveals that it is possible to proceed breeding cluster by cluster. |
本系統中英文摘要資訊取自各篇刊載內容。