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- Limited Genetic Diversity in the HLA Class Ⅱ DP Region of the Atayal Tribe, with Dominant Alleles DPA1*02022 and DPB1*0501 by High Resolution Sequencing Based Typing
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題名 | Limited Genetic Diversity in the HLA Class Ⅱ DP Region of the Atayal Tribe, with Dominant Alleles DPA1*02022 and DPB1*0501 by High Resolution Sequencing Based Typing=使用高解析度的DNA定序法探討泰雅族人的HLA-DP型:以DPA1*02022及DPB1*0501型為主的頻率分佈 |
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作者 | 吳紹基; 蕭育民; 蕭正光; 巫冠毅; 李建瑛; Wu, Shaokee; Shiao, Yu-ming; Shaw, Cheng-kuang; Wu, Kuan-yi; Lee, Chen-ing; van der Zwan,Anne-wil; Tilanus,Marcel G. J.; |
期刊 | 慈濟醫學 |
出版日期 | 19990300 |
卷期 | 11:1 1999.03[民88.03] |
頁次 | 頁15-23 |
分類號 | 362.6 |
語文 | eng |
關鍵詞 | HLA-DPA與HLA-DPB區域; 連鎖不均衡; DNA定序法的HLA定型; 泰雅族; 等位基因及單倍體頻率; HLA-DPA and -DPB region; Linkage disequilibrium; Sequencing-based HLA typing; Atayal tribe; Allele and haplotype frequencies; |
中文摘要 | 人類的白血球抗原( HLA )基因是已知的功能基因中最具多態性的基因群。在非 親屬間的器官與骨髓移植時, 供體與受體間 HLA 配型成功的可能性乃取決於供體資料庫的 大小。 而所需資料庫的大小又決定於該族群 HLA 基因多態性的複雜程度。在本文中,我們 使用 DNA 定序法( SBT )分析花蓮縣秀林鄉泰雅族人的 HLA-DP 基因。先從六十位泰雅族 人的血液細胞抽取 DNA, 然後以 DNA 定序法定出 DPA1 與 DPB1 基因具有變異多態性的第 二個表現序列。 在 DPA1 方面, 發現有三個等位基因:其有 02022 型有最高的頻率( 59 %),跟著是 0103 ( 17 %)及 02011 ( 9 %)兩型。在 DPB1 方面,發現有五個等位 基因:其中 0501 型有最高的頻率( 75 %),跟著是 0310 ( 9 %), 1401 ( 7 %) , 02012 ( 6 %)及 1301 ( 2 %)型。 用最大可能性法可推論出五種雙基因位( two-locus )的單倍體( haplotype ): DAP1*02022-DPB1*0501 ( 57.7 %), DPA1*0103-DPB1*0301 ( 9.2 %), DPA1*02011-DPB1*1401 ( 7.5 %), DPA1*0103-DPB1*02012 ( 5.8 %)及 DPA1*02011-DPB1*1301 ( 1.7 %)。 這些結果顯 示: 泰雅族人的 DPA1 與 DPB1 基因可能具有連鎖不均衡的特性。 最引人注意的乃是高達 55 %的人可能是 DPA1*02022 與 DPB1*0501 的純合子型, 泰雅族人似乎僅有較簡單性的 HLA-DP 多態性。 |
英文摘要 | The polygenic HLA (human leukocyte antigen) gene complex is the most polymorphic cluster of functional human genes known. The degree of HLA polymorphism in an ethnic group determines the size of a donor registry for finding an HLA-matched unrelated donor for solid organ or bone marrow transplantation. In this report, we use sequencing based typing (SBT) to analyze the degree of polymorphism of the class Ⅱ HLA-DP genes from Atayal tribe members living in the Hualien county (Taiwan) at 121 ° East and 24 ° North. DNA was isolated from peripheral blood cells from 60 Atayal individuals. HLA-DPA1 and -DPB1 alleles were identified by sequencing the polymorphic second exon. Three alleles were found for the DPA1 locus, among which 02022 had the highest allele frequency (59%), followed by 0103 (17%) and 02011 (9%). Five alleles were found for the DPB1 locus, with 0501 having the highest allele frequency (75%), followed by 0301 (9%), 1401 (7%), 02012 (6%) and 1301 (2%). Five two-locus haplotypes were inferred by a maximum-likelihood method: DPA1*02022-DPB1*0501 (57.7%), DPA1*0103-DPB1*0301 (9.2%), DPA1*02011-DPB1*1401 (7.5%), DPA1*0103-DPB1-02012 (5.8%) and DPA1*02011-DPB1*1301 (1.7%) . These results suggest that there is linkage disequilibrium within the HLA-DP region in the Atayal tribe. Remarkable is the high frequency (55%) of individuals potentially homozygous for both DPA1*02022 and DPB1*0501 alleles. The Atayal tribe seems to show a very limited HLA-DP polymorphism. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。