頁籤選單縮合
題名 | Identification of Heterogeneous Nuclear Ribonucleoprotein K (hnRNP K) as a Repressor of C/EBP β-mediated Gene Activation |
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作 者 | Miau,Luo-hwa; Chang,Ching-jin; Shen,Bin-jon; Tsai,Wen-hai; Lee,Sheng-chung; | 書刊名 | 中央研究院生物化學研究所論文集 |
卷期 | 24 1998[民87.] |
頁次 | 頁241-248 |
分類號 | 361.19 |
關鍵詞 | Heterogeneous nuclear ribonucleoprotein K; hnRNP K; C/EBP β-mediated gene activation; |
語文 | 英文(English) |
英文摘要 | Transcription factor C/EBPβ has been known to regulate a wide array of genes including those involved in the acute-phase response. One of the molecular mechanisms underlying transcription activation by C/EBP β is through protein-protein interaction with other trangcription factors. Here we report the identification and characterization of physical and functional interactions between C/EBP β and heterogeneous nuclear ribonucleoprotein (hnRNP) K. This interaction results in the repression of C/EBP β -dependent trans-activation of the agp gene. Footprinting assays indicate that hnRNP K cannot bind to the promoter region of agp gene or interfere with the binding of C/EBP β to its cognate DNA site. Furthermore, agp gene activation by the synergistic interaction ofNoppl40 and C/EBP β is abolished by hnRNP K. The kinetics of appearance of C/EBP β -hnRNP K complex in the nuclear extract after initiation of acutephase reaction indicates that hnRNP K functions as a negative regulator of C/EBP β -mediated activation of agp gene. |
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