頁籤選單縮合
題名 | Screening and Cultivatin of a Yeast Strain with High Organoselenium Yields=高有機硒產率酵母菌之篩選與培養 |
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作者 | 劉嘉哲; 鄭文玲; 陳力騏; 楊博文; | 書刊名 | 臺灣糖業研究所研究彙報 |
卷期 | 162 1998.12[民87.12] |
頁次 | 頁51-63 |
分類號 | 368.87 |
關鍵詞 | 酵母菌; 硒酵母; 有機硒; Yeast; Selenium yeast; Organoselenium; |
語文 | 英文(English) |
中文摘要 | 由不同酵母菌(包括Saccharomyces cerevisiae and Candida utilis)菌株中篩選具高有機硒產率之菌株。在對無機硒的耐受程度測試中發現,其中一株S. cerevisiae TSL-1在添加亞硒酸鈉之固態培養基上對硒耐受程度最高。於液態培養環境下,當最初無機硒濃度為20μg/ml時,S. cerevisiae TSL-1在24小時內可累積有機硒達每克乾酵母含1150 μg硒。在無機硒濃度為20μg/ml以內,有機硒最終含量隨培養機中所添加無機硒的量增加而增加。液態培養之硒酵母其有機硒之累積主要在指數生長期,進入停滯期後有機硒之累積顯著減慢甚至完全停止。 |
英文摘要 | Various yeast strains, including Saccharomyces cerevisiae and Candiad utilis, were screened to search for high producers of organoselenium. A strain of S. cerevisiae, strain TSL-1, was found to be able to accumulate up to 1150μg putative organoselenium per gram of dried cells within 24 hours in a medium containing 20μg/ml selenium in the form of sodium selenite. The measured final organoselenium content in suspension cultures increased with the quantity of inorganic selenium initially added to the cultures within 20μg/ml of selenium. The accumulation of selenium in yeast mainly occurred during the exponentially growing phase and significantly slowed down or totally stopped after entering the stationary phase in suspension cultures. |
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