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題 名 | Characteristics of Spontaneous Ca[fec7] Oscillations in Bovine Aortic Endothelial Cells in Ca[fec7]-Free Solution |
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作 者 | Yang,Jung-mou; | 書刊名 | 中國生理學雜誌 |
卷 期 | 41:3 1998.09[民87.09] |
頁 次 | 頁145-150 |
分類號 | 437.22 |
關鍵詞 | Endothelial cell; Ca[fec7] oscillation; ATP; |
語 文 | 英文(English) |
英文摘要 | In nominally Ca -free solution, the incidence of spontaneous Ca oscillations in the cultured bovine aortic endothelial (BAE) cells many occur during superfusion. Application of 3 uM ATP induced a single Ca transient regardless of the prior absence or presence of spontaneous oscillations. The total mobilized [Ca] depends on the incidence of spontaneous oscillations and the length of exposure in Ca -free solution. The maximal rate of rise of the transient in cells induced by ATP immediately after an spontaneous spike was greater than that in cells without any spontaneous activity. But there was no difference in the recovery time constant. The sum of areas of spontaneous and ATP-induced transients in cells with spontaneous activity was not different from that of ATP-induced transient in cells without spontaneous activity. The results suggest that BAE cells, after growth to confluence, are subjected to spontaneous oscillations during superfusion in Ca -free solution. The release of internal Ca in response to ATP is enhanced in cells with spontaneous oscillations. The content of ATP-sensitive stores continually decreased when cells were superfused with Ca -free solution, and most of the spontaneous release of Ca was lost and not sequestrated back into ATP-sensitive stores. |
本系統中英文摘要資訊取自各篇刊載內容。