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題 名 | 白雲杉白色與綠色癒合組織的差異性=Differences between White and Green Megagametophyte Callus in Picea Glauca (Moench) Voss |
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作 者 | 朱紀實; | 書刊名 | 國立臺灣大學農學院實驗林研究報告 |
卷 期 | 12:4=222 1998.12[民87.12] |
頁 次 | 頁299-307 |
分類號 | 436.259 |
關鍵詞 | 癒合組織; 原色體; 葉綠體; 同位酵素; DNA甲基化; Callus; Proplastid; Chloroplast; Isozyme; DNA methylation; |
語 文 | 中文(Chinese) |
中文摘要 | 將白雲杉雌配子體母細胞白色癒合組織與從白色癒合組織誘導生出之綠色癒合組 織, 培養在 MS 培養基( pH 5.8 ), 加上 10 μ M 的 2,4-D 和 BAP,2% 蔗糖及 0.6% 洋菜膠中,皆能維持原有之白色及綠色。分析此二種癒合組織,除了在葉綠素有差別外,綠 色瘉合組織的乾重及蛋白質總量皆較高。以五種與醣類新陳代謝及電子傳遞有關的同位酵素 isocitrate dehydrogenase (IDH), aspartate aminotransferase (AAT), diaphase (DIA) , shikimate dehydrogenase (SKDH) 和 glucose-6-phosphate dehydrogenase (G6PDH) 分析, 顯示白色癒合組織較綠色癒合組織除了在細胞質,粒線體( mitochondria )或葉綠體( chloroplast )中會表現的 IDH, AAT,DIA 及 G6PDH 等皆至少缺一個以上 之同位酵素帶,而只在葉綠體內表現之 SKDH 則並未在白色癒合組織中發現。另外,綠色癒 合組織並不執行光合作用,在同位酵素 DIA 分析,也顯示綠色癒合組織較針葉缺少一 DIA 帶。以對 DNA 甲基化敏感之限制酵素( Hpa Ⅱ, Msp Ⅰ和 Nci Ⅰ)分析,兩種組織 DNA 片段均大於 12 kb,且綠色癒合組織 DNA 片段較針葉 DNA 片段平均為大,顯示綠色癒合組 織 DNA 比針葉 DNA 有較高之甲基化現象。 |
英文摘要 | Incubating white and green megagametophyte calli, induced from white megagametophyte callus, of white spruce on MS medium (pH 5.8) supplemented with 10 μM 2,4-D and BAP, 2% sucrose and 0.6% agar, both calli maintained white and green color. White calli differ from green calli in the lack of chlorophyll and a decrease in dry weight and protein concentration. Differences are also found in isozymes, which regulate the carbohydrate metabolism or electron transport. Five isozymes: isocitrate dehydrogenase (IDH), aspartate aminotransferase (AAT), diaphase (DIA), shikimate dehydrogenase (SKDH) and glucose-6-phospohate dehydrogenase (G6PDH)] were used for analysis. White callus was at least deficient of one locus of the following isozymes: IDH, AAT, DIA and G6PDH, which express in the cytoplasm, mitochondria or chloroplast. The isozyme: SKDH, which only expresses in chloroplast, was not found in white callus. The green callus did not showed any photosynthetic activity. In comparison with needlies, green callus was lack of one IDH locus. Furthermore, the total DNA of green callus and needles was digested with DNA methylation sensitive enzymes: HpaⅡ, MspⅠ and NsiⅠ. The average length of DNA fragment of both samples was greater than 12 kb. However, the DNA fragment of green callus was longer than that of needles. It indicates that total genomic DNA of green callus was more methylated than that of needles. |
本系統中英文摘要資訊取自各篇刊載內容。