頁籤選單縮合
題 名 | Nifedipine, Verapamil and Diltiazem Block Shock-wave-induced Rises in Cytosolic Calcium in MDCK Cells |
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作 者 | Jan,Chung-ren; Chen,Wei-chuan; Wu,Sheng-nan; Tseng,Ching-jiunn; | 書刊名 | 中國生理學雜誌 |
卷 期 | 41:4 1998.12[民87.12] |
頁 次 | 頁181-188 |
分類號 | 418.22 |
關鍵詞 | MDCK cells; Shock waves; Ca[fec7] entry blocker; Fura-2; Calcium imaging; |
語 文 | 英文(English) |
英文摘要 | Nifedipine and verapamil have been shown previously to protect against renal function alterations induced by shock wave lithotripsy (SWL) in humans and rats; however, the mechanism is unclear. This study was aimed to examine whether these drugs could protect cultured kidney cells following shock wave exposure (SWE). The effect of nifedipine, verapamil and diltiazem on Madin Darby canine kidney (MDCK) cells following SWE was examined by determining the release of glutamate oxalactate transferase (GOT) and lactate dehydrogenasse (LDH) in cell suspensions; and also cytosolic Ca□ concentration ([Ca□]□). Immediately after SWE, there was a transient release of GOT and LDH (16% and 4 fold, respectively). In contrast, [Ca □]□ measured within 1-6 hr after SWE gradually increased by 15-156%. The Ca□ entry blockers (1 or 10 μM) failed to inhibit the enzyme release; however, they abolished the progressive rises in [Ca□]□. The Ca□ entry blockers may protect the cells from damage of SWE via maintaining a low resting [Ca□]□. |
本系統中英文摘要資訊取自各篇刊載內容。