頁籤選單縮合
題名 | Determination of Xanthine Bronchodilators in Adulterated Chinese Herbal Preparations by High Performance Liquid Chromatography |
---|---|
作者 | 顧祐瑞; 溫國慶; 何禮剛; 張永勳; | 書刊名 | 中華藥學雜誌 |
卷期 | 50:6 1998.12[民87.12] |
頁次 | 頁337-350 |
分類號 | 414.5 |
關鍵詞 | HPLC; Theophylline; Diprophylline; Traditional chinese medicine; Adulterant; |
語文 | 英文(English) |
英文摘要 | Five xanthine bronchodilators, caffeine, diprophylline, proxyphylline, theobromine and theophylline, were simultaneously assayed in traditional Chinese medicine by high performance liquid chromatography on a Merck RP-select B column. The chromatographic method was carried out using isocratic elution with methanol and 1% acetic acid (15:85, v/v). 8-Chlorotheophylline was used as an internal standard and detection was at 270 nm. Calibration curves of the five adulternants were constructed in the range 8-160 μ g/mL for theobromine and 10-200 μ g/mL for the others. Their correlation coefficients ranged from 0.99993 to 0.99997. Extracts were treated by Supelclean C-18 before HPLC analysis. The recoveries of the adulterants in three traditional Chinese medicinal prescriptions were also studied. Diprophylline and/or theophylline were found and assayed from traditional Chinese medicines by combined solid-phase extraction and HPLC. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。