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題 名 | 骨髓基質細胞的成骨特性=Osteogenic Characterization of Bone Marrow Stromal Cells |
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作 者 | 董醒任; 藍文謙; 楊世芳; | 書刊名 | 中華牙醫學雜誌 |
卷 期 | 18:3 1999.09[民88.09] |
頁 次 | 頁175-190 |
分類號 | 416.93 |
關鍵詞 | 骨髓基質細胞; 細胞形態; 細胞大小; 鹼性磷酸脢; 體外鈣化; Bone marrow stromal cells; Cell morphology; Cell size; Alkaline phosphatase; In vitro mineralization; |
語 文 | 中文(Chinese) |
中文摘要 | 骨髓基質細胞是一種潛力的細胞,它擁有分化形成各種細胞的能力。在骨癒合時 ,骨髓基質細胞可分化形成新骨。本研究目的探討骨髓基質細胞的特性,諸如形態、增生 、鹼性磷酸脢活性,並建立體外鈣化模型,以利吾人在骨細胞生物學的研究。研究方法是 以細胞培養的方式將大鼠骨髓細胞加以分離;在細胞近飽和時固定,並用光學顯微鏡與掃 描式電子顯微鏡觀察細胞形態。使用細胞顆粒計數器以分析細胞數量與大小分佈,並以免 疫化學染色技術觀察integrin的表現。此外也以鹼性磷酸脢(alkaline phosphatase),以 及 von Kossa 與 alizarin red 染色法分析骨髓基質細胞之分化及鈣化。結果發現骨髓基 質細胞是由許多不同形態、大小不一的細胞所組成。培養一週後,細胞小大是以 10-20 μ m 為主,並有鹼性磷酸脢的正反應與 integrin α□、α□的表現。此外本研究並成功建立 了大白鼠骨髓基質細胞體外鈣化模型。這些發現增加吾人對骨髓基質細胞形態、分化、鈣化 等生物特性的了解。大白鼠骨髓基質細胞鈣化模型由於動物取得容易且鈣化所需時較短。更 為研究生長因子對於骨組織再生調控,提供了一個絕佳的體外模型。 |
英文摘要 | During bone healing, bone marrow stromal cells may re-establish the osteoblast phenotype and induce new bone formation. The purpose of this study was to characterize the morphology, proliferation, α□ and α□ integrin expression, alkaline phosphatase activity, and in vitro mineralization of bone marrow stomal cells. The bone marrow was flushed from rat femurs. The adherent cells were separated from the non-adherent cells, and maintained in α -minimal essential medium (MEM) containing 10% fetal bov ne serum (FBS) and ascorbic acid. Changes in cell size and distribution were evaluated by a particle size and distribution counter.Morphological changes in bone marrow stromal cells were evaluated by light and electron microscopes. The α□, α□ integrin and fibronectin expression were evaluated by indirect immuno-peroxidase localization using specific antibodies under light and laser confocal scanning microscopes. In an in vitro mineralization model,10 □ M dexamethasone and 10 mM β -glycerophosphate were u sed. The formation of mineralized nodules was evaluated using von Kossa and Alizarin red stains. Results indicated that the number of cells of 5-10 μ m-decreased with time for all treatments. Scanning electron microscopic examinations also demonstrated a heterogeneous morphotype of bone marrow stromal cells. An in vitro mineralization model of rat bone marrow stromal cells was also successfully developed. In conclusion, results from the present study enhance our understanding of the biological characteristi cs of rat bone marrow stromal cells and provide an excellent in vitro model for further evaluation of the impact and molecular mechanisms of growth factors on bone mineralization. |
本系統中英文摘要資訊取自各篇刊載內容。