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題 名 | 電擊融合對植物原生質體周邊微管網之傷害 |
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作 者 | 蔡珣珣; | 書刊名 | 中華農學會報 |
卷 期 | 154 1991.06[民80.06] |
頁 次 | 頁14-20 |
分類號 | 371.8 |
關鍵詞 | 周邊; 原生質體; 植物; 傷害; 微管網; 電擊融合; |
語 文 | 中文(Chinese) |
中文摘要 | 原生質體具備一個完整且緻密的周邊微管網,被認為是完成正常細胞分裂所必須。缺少或帶有不夠緻密之微管網的細胞,可以修補並重新建立一個完整的微管網以促使細胞分裂,然而由於修復所需要的時間,分裂往往延遲進行。本研究由免疫螢光染色技術,觀察到電極融合處理對原生質體的周邊微管網明顯地造成傷害。這個結果解釋了電極融合技術經常遭遇到的困難與失敗的原因,包括,經電擊融合的雜交細胞比較未經融和處理或經化學融合者有低的細胞分裂頻率,以及首次細胞分裂經常延遲1~2天進行等。本研究更首度發現「液胞微管網」的存在,它的功能尚不清楚,推測可能與帶大液胞的細胞仍能行正常分裂,或者液胞的「分室」特性有關。 |
英文摘要 | In Nicotiana the presence of a cortical microtubular lattice of certain density is a prerequisite for protoplast division. If such a net is missing or to wide-meshed in the protoplast donor tissue, it must be reestablished which causes a delay in cell division. Cell wall regeneration, which also must precede division, occurs simultaneously with the formation of the randomly structured lattice. During polyethylene glycol-induced fusion of isolated protoplasts the cortical microtubular lattices retain their pattern, integrate into each other and cell division in somatic cell hybrids proceeds without time delay. Often cytokinesis is even expedited in hybrid cells due to a heterosis-like effect. However, electrofused protoplasts exhibit dramatic damages of the cortical microtubular net when observed with immunofluorescence microscopy. The damaging effect on the cytoskeleton caused by the drastic electrical treatment applied in electrofusion can explain the limited success of this technique in producing dividing hybrid cells. It can also account for the delay in cell division which is usually observed in electrofused protoplasts as compared to the parental cells or to PEG-induced hybrids. The additional time seems to be necessary to repair the perforated net. While studying the fate of cortical microtubules during protoplast fusion we found evidence for the existence of a dense microtubular lattice bordering the vacuole. The function of such a lattice remains unknown but it could be related to the ability of highly vacuolated cells to carry out almost equal divisions and/or to the compartmentation which has been described to exist inside the vacuole. |
本系統中英文摘要資訊取自各篇刊載內容。