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題名 | 牛、兔、雞、鴨、鵝和老鼠等之精子鑽入鹽化豬卵透明帶的探討=The Use of Salted Porcine Ova for Sperm Penetration Assessment for Cattle, Rabbit, Chicken, Duck, Goose and Rat |
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作者 | 吳明哲; 李秀美; 黃祥吉; | 書刊名 | 畜產研究 |
卷期 | 31:2 1998.06[民87.06] |
頁次 | 頁137-151 |
分類號 | 437.21 |
關鍵詞 | 家畜; 家禽; 老鼠; 透明帶; 鹽化卵; 精子; 粘著力; Livestock; Poultry; Rat; Zona pellucida; Salted oocyte; Sperm; Binding ability; |
語文 | 中文(Chinese) |
中文摘要 | 豬卵巢濾泡中的卵丘卵母細胞複合體經鹽化保存於冰箱,直至使用前才予以脫 鹽和去除卵丘細胞層,使卵透明帶裸露o脫鹽後的豬卵每15個放入一培養小滴.每一培養皿 內有兩個培養小滴。稀釋後糟液再置放入這兩個培養小滴,使之和卵混合,蓋上礦物油的 培養小滴於39℃恆溫培養箱中培養15小時。拈附在豬卵透明帶外的多餘精子經反覆沖吸方 式洗掉,再把豬卵螢光染色,鐃檢透明帶上的精子數。本試驗共計39頭動物的精液被測試 ,分別有五頭紐西蘭白兔、五隻土雞、四隻菜鴨和九隻正番鴨的新鮮精液,一頭老鼠的附 睪尾部精液,以及有七頭荷蘭乳牛、四頭台灣水牛和四隻中國鵝的冷凍精液。在鏡檢的1 40個豬卵記錄裡,單一豬卵透明帶中能被乳牛、水牛、兔、雞、菜鴨、正番鴨、鵝、老鼠 等之精子鑽入最多的精子數分別有69、118、15、42、39、69、13、0個精子。當比較同品 種不同個體精液之精子鑽入豬卵透明帶的比率或卵透明帶中的精子數,均顯示有種公畜個 體差異存在。老鼠精子雖可粘附在鹽化豬卵的透明帶上,但並未鑽入o因此,以鹽化豬卵 來測試其他家畜禽精子之鑽入透明帶的能力是有其潛在用途。 |
英文摘要 | The penetration of sperm into the zona pellucida is a major process of fertilization. Porcine ova were refrigerated in salt solution and the salt-stored ova were washed prior to their usage. Groups of 15 desalted ova were transferred into a droplet of fertilization 'medium and two droplets in a culture dish were prepared. Sperm suspension were added into both droplets, i.e., 30 ova as an assessment unit for each dosage of the testing semen. Droplets in petri dish were then covered with mineral oil and incubated at 3TC for 15 hours. After incubation, loosely binding sperm were washed away from the surface of the zona pellucida and then examined under a fluorescent microscope for the number of penetrated sperm. A total of 39 semen samples consisting of frozen semen from seven Holstein bulls, four Taiwan water buffalo bulls and four Chinese geese, epididymal semen collected from cauda epididymis of a rat, and fresh semen from five New Zealand White rabbits, five Taiwan native chickens, four Tsaiya ducks and nine Muscovy ducks, was assessed for the penetration ability of sperm. Records of 1540 ova with the number of penetrated sperm revealed that the highest number of sperm penetrated into the zona pellucida were 69, 118, 15, 42, 39, 59, 13, and 0, respectively by dairy cattle, water buffalo, rabbit, chicken, tsaiya duck, muscovy duck, goose, and rat. It implied that salt-stored porcine eggs could be used for the assessment of penetration ability of sperm into the zona. Comparison of penetration results of various individuals within each species, either by counting number of ova penetrated or by counting number of sperm penetrated into the zona, showed that the variation among male individuals existed. Rat sperm bound onto the zona pellucida of salted ova but it did not bind onto the zona of a fresh and cumulus-free ova. Therefore, the use of porcine ova for the assessment of fertility of other farm species is feasible. It could be applied to identify the fertile male from stocks in a short time. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。