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題名 | La[fec4]狇Inhibits the UTP-Induced Ca[feb4]狇Mobilization in MDCK Cells |
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作者姓名(外文) | Jan,Chung-ren; Ho,Chin-man; Wu,Sheng-nan; Huang,Jong-khing; Tseng,Ching-jiunn; | 書刊名 | 中國生理學雜誌 |
卷期 | 41:1 1998.03[民87.03] |
頁次 | 頁59-64 |
分類號 | 397 |
關鍵詞 | La[fec4]狇; Ca[feb4]狇signaling; UTP; MDCK cells; Fura-2; |
語文 | 英文(English) |
英文摘要 | We have studied the effects of La□ on UTP-induced rises in intracellular calcium levels ([Ca□]□) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. UTP induced Ca□]□ rises dose-dependently with an EC□ of 1 mM. The Ca□ signal was triggered by a Ca□ release from the inositol 1,4,5-trisphosphate (IP□)-sensitive pool because the signal was completely blocked by pretreatment of the endoplasmic reticulum (ER) Ca□ pump inhibitor thapsigargin (TG) or the phospholipase C (PLC) inhibitor U73122. Both the peak height and under-curve area of 10 μM UTP-induced Ca□ signal was reduced by approximately 40% by extracellular Ca□ removal, suggesting that UTP induced capacitative Ca□ entry. La□ inhibited the UTP-induced Ca- signal dose-dependently when added before or after UTP. Pretreatment of 0.1 mM La□ inhibited the UTP response more than Ca□ removal did. The mechanisms underlying the La□ inhibition appear to involve not only block of capacitative Ca□ entry. |
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