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題 名 | Corynebacterium glutamicum在高濃度鹽份培養基脯胺酸發酵之研究=Proline Fermentation of Corynebacterium Glutamicum in Media Containing a High Concentration of NaCl |
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作 者 | 李根永; 李孟修; | 書刊名 | 中國農業化學會誌 |
卷 期 | 36:1 1998.02[民87.02] |
頁 次 | 頁57-64 |
分類號 | 369.34 |
關鍵詞 | 脯胺酸發酵; 滲透壓; 回應曲面法; Proline fermentation; Osmotic pressure; Response surface methodology; |
語 文 | 中文(Chinese) |
中文摘要 | Corynebacterium glutamicum在含10%氯化鈉的培養基中進行脯胺酸發酵,菌體 的生長雖然會受壓抑,但脯胺酸的濃度卻高於無添加鹽分對照組的三倍。 而在 3 %氯化鈉 培養基中,則無此效果。 經 8 %氯化鈉培養基馴養的菌體,移入不含氯化鈉的培養基,以 解除氯化鈉施予的滲透壓刺激,結果發酵不利於脯胺酸的產生。此現象意味著必需在高滲透 壓力下,才有利於菌體生合成脯胺酸。為尋求氯化鈉、氮源(氯化銨)、及碳源(葡萄糖) 於培養基中的最佳組合, 利用中心混成實驗設計, 並以回應曲面法( Response surface methodology )統計分析,結果求得其濃度分別為 6.90 %、 1.40 %及 1.76 %,在此條 件下, 脯胺酸的產量是 0.125 ± 0.045 μ mole ╱ mL,和預估值 0.123 μ mole ╱ mL 比較,經 t ﹣檢試統計分析,兩者間在 5 %水準下無顯著差異。 |
英文摘要 | The proline yield of Corynebacterium glutamicum in fermentation media containing 10% NaCl was 3 times higher than that of the control although the growth of bacteria was depressed. The media containing a lower concentration of NaCl (3%) did not show the same effect. The bacteria tamed by 8% NaCl lost the ability to produce proline if the bacteria were switched to regular media to release osmotic stress. This revealed that osmotic pressure caused by the high concentration of the solute could stimulate the biosynthesis of proline. The optimum concentrations of NaCl, nitrogen source (NH �� Cl) and carbon source (glucose) in the medium were 6.90%, 1.40% and 1.76%, respectively, which were determined by RSM (response surface methodology) analysis using a central composite experimental design. Under these conditions, the production of proline was 0.125 ± 0.045 μ mole/mL, and no significant difference at the 5% level was detected by t-test analysis compared with the predicted value (0.123 μ mole/mL). |
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