頁籤選單縮合
題 名 | Separation of Oligo/Polymers of 5-N-Acetylneuraminic Acid, 5-N-Glycolylneuraminic Acid, and 2-Keto-3-Deoxy-D-Glycero-D-Galacto-Nononic Acid by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detector |
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作 者 | Zhang,Ye; Inoue,Y.; Inoue,S.; Lee,Y. C.; | 書刊名 | 中央研究院生物化學研究所論文集 |
卷 期 | 23 1997[民86.] |
頁 次 | 頁211-217 |
分類號 | 361.4 |
關鍵詞 | |
語 文 | 英文(English) |
英文摘要 | A sensitive and efficient method to analyze oligo/poly-sialic acids containing α2-8-linked 5-N-acetyl-neuraminic acid (Neu5Ac), 5-N-golycolylneuraminicacid (Neu5Gc), and deaminated neuraminic acid (KDN) using high-performance anion-exchange chro-matography (HPAEC) with a pulsed amperometric detector (PAD-2) has been developed. Using a CarboPac PA-100 column and sodium nitrate as the pushing agent, polymers in colominic acid with degree of poly-merization (DP) up to 80 were separated in 68 min. A similar DP. Based resolution was also obtained on a CarboPac PA-1 column. The elution ladders of the Neu5Ac, Neu5Gc, and KDN series were sufficiently different to be used as diagnostic indices. This technique was applied to identification of the sialic acid compoments in a polysialoglycoprotein (PSGP) sample as well as monitoring the oligo/poly-KDN-containing fractions during the purification of KDN-containing glycoprotein (KDN-gp). The maximum DPs of oligo-Neu5Gc and oligo-KDN that can be detected in PSGP and KDN-gp hydrolysates were 11 and 8, respectively. The high sen-sitivity of this method was demonstrated by the quantification of Neu5Ac oligomers. Distributions of the monomer and oligo/polymers in the acid and enzymatic hydrolysates of colominic acid and PSGP under different conditions were also studied. |
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