頁籤選單縮合
題 名 | Purification and Cloning of Carp Nephrosin, a Secreted Zinc Endopeptidase of the Astacin Family |
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作 者 | Hung,Ching-hsiang; Huang,Hon-ren; Huang,Chang-jen; Huang,Fore-lien; Chang,Geen-dong; | 書刊名 | 中央研究院生物化學研究所論文集 |
卷 期 | 23 1997[民86.] |
頁 次 | 頁159-165 |
分類號 | 361.4 |
關鍵詞 | |
語 文 | 英文(English) |
英文摘要 | We have purified a secreted proteinase of 23 kDa from carp head kidney by sequential column chromatography on a Reactive Blue 72-agarose dye affinity column and an FPLC Mono-P column. The secretion of this proteinase from carp head kidney can be stimulated by high concentrations of potassium. Since the carp proteinase is present mainly in the head kidney, kidney, and spleen (all of which are lymphohematopoietic organs), it is named nephrosin. The carp nephrosin is most sensitive to metal chelators, but not to inhibitors specific for other classes of proteinases. A cDNA clone has been isolated from a carp head kidney Cdna library by immunoscreening with a polyclonal antiserum raised against purified nephrosin. The cloned cNDA is 1086 base pairs in length and has an open reading frame encoding a protein of 273 amino acids, including a 19-amino acid signal peptide and 56-amino acid propep-tide. The deduced amino acid sequence shows moderate levels of identity to medaka HCE1 (52.5%), medaka LCE (50.7%), CRAYFISH ASTACIN (33.2%), murine meprina-α(34%), and murine meprin-β(33.5%), all members of the astacin family of zinc endopeptidases. Nephrosin is the first member of the astacin family found in lymphohematopoietic tissues. |
本系統中英文摘要資訊取自各篇刊載內容。