頁籤選單縮合
題名 | Molecular Cloning, Structural Analysis, and Expression of Carp ZP2 Gene |
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作者姓名(外文) | Chang,Y. S.; Hsu,C. C.; Wang,S. C.; Tsao,C. C.; Huang,F. L.; | 書刊名 | 中央研究院生物化學研究所論文集 |
卷期 | 23 1997[民86.] |
頁次 | 頁39-48 |
分類號 | 361.4 |
關鍵詞 | Carp; Egg membrane; ZP2; Genomic structure; Expression; |
語文 | 英文(English) |
英文摘要 | The cDNAs encoding carp ZP2 homologous to winter flounder and mammalian ZP2 were cloned. Carp ZP2 contains a tandemly repetitive domain and a nonrepetitive domain. A repeat is composed of 13 amino-acid residues whose consensus sequence is QQTSQQFQPQKPAN. The length of the repetitive domain is highly variable, but that of the nonrepetitive domain is fairly constant among various cDNAs. The termination codons of various cDNAs appear at three different positions. Three groups of cDNAs were therefore categorized. Groups I-III encode a nonrepetitive domain of 356, 255, and 10 residues, respectively. A carp ZP2 gene corresponding to group II cDNAs was cloned. It spans 2.4 kb and consists of eight exons and seven introns. Carp ZP2 mRNA was detected only in oocytes but not in other tissues. Carp ZP2 is heterogenous in size. The molecular weight ranges from 40-80 kDa. It is present in vitellogenic but not in previtellogenic oocytes, nor in other tissues. Carp ZP2 content in oocytes increases as vitellogenesis proceeds. |
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