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題 名 | 利用對Desferrioxamine的感受性及Trehalose之不發酵性鑑定Staphylococcus Epidermidis=Identification of Staphylococcus Epidermidis by Desferrioxamine Susceptibility and Trehalose Fermentation Tests |
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作 者 | 邱明億; 王梓明; 張政雄; 吳俊忠; 蔡文城; | 書刊名 | 中華民國微生物及免疫學雜誌 |
卷 期 | 30:4 1997.11[民86.11] |
頁 次 | 頁274-280 |
分類號 | 414.83 |
關鍵詞 | Staphylococcus epidermidis; Susceptibilith to desferrioxamine; Identification; |
語 文 | 中文(Chinese) |
中文摘要 | 表皮葡萄球菌(Staphylococcus epidermidis)為凝固�t陰性葡萄球菌中最常分離的菌種。雖然以細菌對desferrioxamine之怠受性及對trehalose之不發酵性的試驗方法曾被用於鑑定 S. epidermidis ,但desferrioxamine 感受性試驗流程所用的培養基或接種試驗菌的量及方法均不符合NCCLS所建議的紙錠瓊脂擴散藥敏試驗方法,因此吾等利用111株臨床庫存的凝固�t陰性葡萄球菌菌株(其中51株為S.cpidermidis 、15株為S.hominis、45株為其它凝固�t陰葡萄球菌)為試驗菌,以Muclier-Hinton agar為試驗培養基,並以NCCLS建議的接種菌量及方法操作DESFERRIOXAMINE 紙錠瓊脂擴散試驗,結果發現desferrioxamine對S.epidermidis及S. hominis 菌株均對1mg desferrioxamine紙錠呈感受性(抑制環直徑大小介於28-37mm)。以瓊脂稀釋試驗方法發現desferrioxamine對S.epidermidis之最低抑菌濃度為125 ug/ml。此兩個菌種可進一步利用trebalose發酵試驗加以區分(S.epidermidis 菌株不會發酵利用,而S.hominis則可)。因此吾等認為檢驗室可配合常規的紙錠瓊脂擴散藥敏試驗方法,利用對1mg desferrioxamine紙錠之敏感性及trchalose 之不發酵特性快速鑑定出凝固�t陰性葡萄球菌中之S. epidermidis。 |
英文摘要 | The importance of coaguasenegative staphyolcocci, especially Staphylococcus eoidermidis in clinical and nosocomial infection are recognized increasingly in recent years. A rapid and accurate identification of S. epidermidis is therefore important and necessary. A new test, susceptibility to desferrioxamine, coupled with trehalose fermentation has been recommended for the identification of this organism. However, the medium and method used are different from what has been recommended by the NCCLS. To investigate the feasibility of using the desferrioxamine susceptibility test in conjuction with the routinely used disc agar diffusion test, we employed 111 staphylococcal strains (including 51 S. epidermidis isolates, 15 S. hominis and 45 other ciagulase-negative staphylococci) as test organisms, and followed the procedures recommended by the NCCLS in which Mueller-Hinton agar and standard inoculum were used. Results indicated that all strains of S. epidermidis and S. hominis were susceptible to 1 mg desferrioxamine (the diameter of the inhibition zone were 28∼37 mm). The minimum inhibitory concentrations of desferrioxamine to S. epidermidis and S. hominis isolates were determined to be 125 ug/ml. Further differentiation of S. hominis and S. epidermidis can be made by their ability to ferment trehalose, the former could while the latter could not. We conclude that the desferrioxamine susceptibility test of coagulase-negative staphylococci can be used in conjuction with the routine disc agar diffusion method. S. epidermidis can be identifid rapidly and accurately by its susceptibility to 1 mg desferrioxamine and inability to ferment trehalose. |
本系統中英文摘要資訊取自各篇刊載內容。