頁籤選單縮合
題 名 | 霧臺柿莖頂組織培養之研究=Studies on Shoot Tip Culture of Wutai Persimmon (Diospyros Japonica Sieb. et Zucc.) in Vitro |
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作 者 | 黃慧美; 楊耀祥; | 書刊名 | 興大園藝 |
卷 期 | 23:1 1998.06[民87.06] |
頁 次 | 頁1-15 |
分類號 | 435.395 |
關鍵詞 | 柿; 莖頂培養; Persimmon; Shoot tip culture; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究以砧木用霧臺柿(Diospyros japonica Sieb. et Zucc.)為試驗材料,探討培養基組成及培養方法對其莖頂培養之影響。在霧臺柿新梢生長旺盛期,採取長約5cm頂部,切取約0.2-0.3mm大小帶2片葉原體的生長點,植入1/2MS添加1mg/l zeatin之固態培養基進行初代培養,經30日後移植於相同配方濃度之固態培養基培養,可以促進培植體生長。培植體經初代培養後移至增殖培養基中培養,以MS添加BA或2ip 5∼10mg/l或zeatin 1mg/l之培養基培養40日後可獲得增殖之枝梢,當增殖倍數低時,可利用添加zeatin 1mg/l 之雙相培養基進行增殖,以提高增殖倍數。經增殖培養所得長20mm之枝梢扦插於含IBA 10mg/l之1/2MS培養基中暗處理培養7日後,移至auxin-free之培養基培養23日,可獲得發根小苗。 |
英文摘要 | Shooot tip culture of thw Wutai persimmon (Diospyros japonica Sieb. et Zucc.) was studied in vitro. Optimal micropropagation conditions for culture establishment, shoot proliferation and rooting were investigated. Shoot tips were harvested about 5 cm in length from actively field grown plants and shoot apices were excised 0.2-0.3 mm for initial culture. The best grown were achieved when shoot apices cultured on solid medium with 1/2 strength of Murashige and Skoog (MS) containing zeatin 1 mg/l, sucrose 30g/l and agar 7g/l for 30 days. These explants were transferred to the same component solid medium for further growth. Multiple shoots were induced by MS medium containing sucrose 30g/l and agar 7g/l plus BA 5mg/l, 10mg/l or 2ip 5mg/l, 10mg/l or zeatin 1mg/l for 40 days. By using double-phase medium culture, the multiplication rate of explant could be increased. For rooting, when shoot (20mm) were placed on 1/2 MS medium wit IBA 10mg/l, and incubated in darkness for 7 days, then transplanted to an auxin-free 1/2 MS medium for 23 days, the rooting percentage, number and length of roots appeared better. |
本系統中英文摘要資訊取自各篇刊載內容。