頁籤選單縮合
題名 | Development of Monoclonal Antibody Against Mycoplasma Gallisepticum=Mycoplasma Gallisepticum單源抗體的研製 |
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作者 | 林茂勇; 黃國榮; 鄔弘盛; 鄭仁宗; |
期刊 | 國立屏東技術學院學報 |
出版日期 | 19950600 |
卷期 | 4 1995.06[民84.06] |
頁次 | 頁1-9 |
分類號 | 361.393 |
語文 | eng |
關鍵詞 | Mycoplasma gallisepticum之單株抗體; Mycoplasma gallisepticum之抗體; Mycoplasma gallisepticum蛋白質之電泳; Monoclonal antibody against mycoplasma gallisepticum; Polyclonal antibody against mycoplasma gallisepticum; Electrophoresis of the protein of mycoplasma gallisepticum; |
中文摘要 | 以標準方法融合BALB/c骨髓瘤細胞和經Mycoplasma gallisepticum(MG)S6株菌 體免疫 BACB/c 的脾細胞開發而成的一種抗單源抗體經確認為 IgG1, 其輕鏈為 Kapa 鍵。 雖然該單源抗體在簡單免疫墨點試驗取稀釋 10,240 倍抗體 5 μ l 和膠內沉澱反應取稀釋 64 倍的抗體尚可得到陽性反應, 并且可用西方墨點試驗檢出為對抗 65Kd 的胞膜蛋白,不 過它卻無法在平板凝集、血球抑制凝集和生長抑制試驗中發生陽性反應。此單源抗體將可利 用來研究 MG 胞膜蛋白抗原。 |
英文摘要 | A monoclonal antilbody against strain S6 of Mycoplasma gallisepticum (MG) was prepared by fusion of FO myeloma cells with splenocytes derived from BALB/c mice and identified as the kappa chain of isotype IgG1 by standard procedures. Although it had a titer of 1: 10, 240 in the simple immunodot-blotting assay with 5 μ l of diluted antiserum, had a titer of 1:64 in the agar gel precipitation and reacted with 65 kilodaltons of the homologous membrane protein in the Western blotting test, it failed to react in rapid plate agglutination, hemagglutination-inhibition and growth-inhibition tests. The monoclonal antibody might be useful for studying the membrane protein antibody of MG. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。