頁籤選單縮合
題名 | The Effect of Lactobacillus Strains in Milk on Lactose Utilization=乳酸桿菌對乳糖利用之影響 |
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作者 | 林美吟; | 書刊名 | 嘉南學報 |
卷期 | 20 1994.11[民83.11] |
頁次 | 頁21-31 |
分類號 | 346.215 |
關鍵詞 | Lactose digestion; Lactobacillus acidophilus; Lactobacillus gasseri; Milk; |
語文 | 英文(English) |
中文摘要 | 本研究以十二患有乳糖不耐症者進行人體試驗,十二位試驗者先後喝 下四組含有乳酸菌的試驗組牛乳及一對照組牛乳。試驗組牛乳中含有10�顏fu/ml 的乳酸桿菌Lactobacillus acidophilus LA-1或Loctobacillus gasseri ADH(為冷凍濃縮 菌種或實驗室培養菌種)。由氫氣濃度結果顯示,含乳酸菌的牛乳稍稍改善(未達 顯著)人體對乳糖的利用(P=0.07 for L.acidophilus LA-1,P=0.36 for L.gasseri ADH),而 且含乳酸菌的牛乳也減經了試驗者的不適症狀,尤其是腹脹情形(P=0.06)。另外 在不同的培養條件下,發現冷凍濃縮的L. acidophilusM LA-1的β-半乳糖���t活 性約為L. gaser ADH的70倍。當以改變糖含量的MRS培養時,活菌的數日LA- 1約為ADH的10倍。而當MRS只含有乳糖時,所培養出來的乳酸菌其β-牛乳 糖���t活性較含葡萄糖與乳糖培養者高4至11倍。此外,由本質驗結果可知. 雖然不同的乳酸菌具有差異極大的β-半牛乳糖���t活性,但可造成類似的乳糖 利用改進效果,因此可推斷β-半牛乳糖���t為乳酸菌必要但非唯一的改善乳糖 利用的特性。 |
英文摘要 | Four lactobacilli-containing milks and a control were fed to 12 lactose maldigesters in a random, blinded protocol. Milks contained 10�� cfu/ml of either LactobacUlus acidophilus LA-1 or LactobaciUus gasseri ADH prepared with commercially obtained frozen or freshly grown cultures. Breath hydrogen (H��) production and symptoms were monitored to evaluate lactose digestion and tolerance. Total H�� production was modestly reduced following consumption of the lactobacilli milks, but the differences failed to reach significance (P=0.07 for LA-1 and P=0.36for ADH). All subjects reported less severe symptoms, primarily flatulence (P= 0.06), followingconsumption of lactobcilli milk. Frozen cultures of LA-1 exhibited a 70-fold higher β-galactosidase (β-gal) activity(per cfu) than frozen ADH. Cell counts were 10 fold higher with LA-1 thanwith ADH, when grown in vitro in modified MRS media. β-gal activities of lactobacilli grown inMRS with only lactose were 4- to ll-fold above enzyme activities in MRS with both glucose andlactoe. The results demonstrate that different levels of β-gal in Lactobacilli-containing milks mayproduce similar improvements in lactose digestion and tolerance. It is likely that β-gal activity isa necessary, but not sufficient attribute for optimizing lactobacilli enhancement of in vivo lactosedigestion. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。