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題 名 | Detection of RNA Polymerase Gene of Foot-and-Mouth Disease Virus (FMDV) from Infected Cell by Polymerase Chain Reaction (PCR)=以聚合酶鏈反應方法從感染口蹄疫病毒的細胞中檢測病毒核醣核酸聚合酶基因 |
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作 者 | 林有良; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷 期 | 60 1992.12[民81.12] |
頁 次 | 頁35-42 |
分類號 | 437.246 |
關鍵詞 | 口蹄疫病毒; 細胞檢測; |
語 文 | 英文(English) |
中文摘要 | 使用三套寡核?苦酸引動子進行聚合?鏈反應仟(PCR)的測試,以放大口蹄疫 病毒的遺傳物質。前二套寡核?酸引動子,是依據口蹄疫病毒A27病毒株的核醣核酸聚合? 基因的核酸序列所設計,在臺灣合成後,帶至美國農業部梅島動物疾病研究中心的實驗室進 行測試,結果至少可以放大八株的口蹄疫病毒,包含五種血清型,依序為A5、A5w、A24、 Cl、C3、SATl、SAT2和SAT3等八株,但對01-Lomb、Ol-Campo及Asia-1等三株口蹄疫病毒則 無法檢測;第二套引動子是用來當做巢式引動子,結果可以成功地鑑定第一套引動子對口蹄 疫病毒遺傳物質放大的產物;第三套引動子,則是依據口蹄疫病毒Cl-Santa Pau(C-S8)病毒 株的核醣核酸聚合?基因的核酸序列所設計而成的,它對使用第一套引動子所不能檢測的三 株病毒進行PCR反應,則可成功地檢出01-Lomb與Asia-l等二種病毒株,但仍無法檢出 0l-Campo此一病毒株。如此,存在於口蹄疫病毒主要血清型與亞型病毒株的核醣核酸聚合? 基因之非功能區的核酸序列間的異源性及此等不同的血清型和亞型病毒,則似乎可以應用 PCR技術加以檢測。 |
英文摘要 | Three sets of oligonucleotide primers were tested for the amplification of the foot-and-mouth disease virus (FMDV) genome by polymerase chain reaction (PCR). The first two sets of oligonucleotide primers, designed synthesized and brought to a United States Department of Agriculture (USDA) laboratory for testing. They were able to amplify at least 8 of the strains of FMDV tested, covering 5 serotypes, including A5, A5W, A24, C1, C3, SAT1, SAT2 and SAT3, but not 01-Lomb, 01-Campo, nor Asia-1. The second set of the primers was uasd as nested primers and successfully identified the nucleotide products of the first set of primers as a copy of the FMDV genome. A third set of primers, based on the nucleotide sequence for the RAN polymerase gene of C1-Santa Pau(C-S8), was used to test 01-Lomb, 01-Campo, and Aisa-1, which had been missed by the first set of primers. These primers successfully detected 01-Lomb and Asia-1, but not 01-Campo. Thus, heterogeneity in the nucleotide sequence for the non-functional portion of RAN polymerase gene exists among the major serotypes and subtypes, and the differences appear to be detectable by the polymerase chain reaction technique. |
本系統中英文摘要資訊取自各篇刊載內容。