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題 名 | Cell Cycle Synchronization of Human T-cell Line MOLT-4 with Thymidine=人類T細胞MOLT-4細胞周期同步化 |
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作 者 | 蔡維明; | 書刊名 | 慈濟醫學 |
卷 期 | 5:2 1993.06[民82.06] |
頁 次 | 頁63-71 |
分類號 | 364 |
關鍵詞 | MOLT4細胞; T細胞; 人類; 同步化; 周期; |
語 文 | 英文(English) |
中文摘要 | 利用胸喀碇將人類MOLT-4T細胞培養周期同步化,研究結果顯示0.5mM 胸喀碇是使細胞同步化最好的濃度。同步化實驗步驟包括三部份:第一步驟,首 先加入胸喀碇浸泡,放置十五小時後洗去溶液;第二步驟,將T細胞放入新鮮培養 基浸泡二十二個小時;第三步驟,再加入胸喀碇溶液浸泡九個小時。結果發現實 驗步驟剛完成之時,約有90的細胞停留於G,/S交界處。MOLT-4細胞由雙重胸嘧啶 阻隔釋放後,其同步化的T細胞於實驗步驟完成後10小時,約有75到S phase,14-15 小時後約有50到G��+Mphase,22小時後約有85回到G�皖hase。 本研究提供雙重胸嘧啶阻隔之最理想作法,討論細胞周期迅速失掉同步化的可能 理由,並且回顧有關哺乳動物細胞周期同步化的文獻。(慈濟醫學 1993;5:63-71) |
英文摘要 | Cultures of the human T-cell line MOLT-4 were synchronized by double thymidine block. Aconcentration of 0.5 mM thymidine was found to be optimal and a synchronization protocol consisting of a 15-hour first thymidine block followed by a 22-hr release and a 9-hour second thymidine block yielded about 90 celts in the G/S boundary. MOLT-4 cells which were releasedfrom the double thymidine block moved through the cell cycle in a synchronized fashion with 75in S phase, 50 in G��+M phase and 85 in �湟 phase at 10, 14-15 and 22 hours after block,respectively. A general scheme for optimal double thymidine block of cell lines in culture waspresented. The likely reasons for the subsequent quick decay of synchronization which is frequentlyobserved following the first cell cycle were discussed. Reports in literature utilizing single or doublethymidine block for mammalian cell synchronization were also reviewed. (Tz'u-Chi Med J 1993;5: 63-71) |
本系統中英文摘要資訊取自各篇刊載內容。