頁籤選單縮合
題名 | ER Ribosomal-binding Protein 1 Regulates Blood Pressure and Potassium Homeostasis by Modulating Intracellular Renin Trafficking= |
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作者 | Chiu, Chu-hsuan; Hsuan, Chin-feng; Lin, Shih-hua; Hung, Yi-jen; Hwu, Chii-min; Hee, Siow-wey; Lin, Shu-wha; Fong, Sitt-wai; Hsieh, Patrick Ching-ho; Yang, Wei-shun; Lin, Wei-chou; Lee, Hsiao-lin; Hsieh, Meng-lun; Li, Wen-yi; Lin, Jou-wei; Hsu, Chih-neng; Wu, Vin-cent; Chuang, Gwo-tsann; Chang, Yi-cheng; Chuang, Lee-ming; |
期刊 | Journal of Biomedical Science |
出版日期 | 20230000 |
卷期 | 30 2023[民112] |
頁次 | 頁(13)1-(13)16 |
分類號 | 415.3 |
語文 | eng |
關鍵詞 | Arrhythmia; Blood pressure; Cardiovascular disease; Hyperkalemia; Hyporeninemic hypoaldosteronism; Renin-angiotensin-aldosterone system; RRBP1; |
英文摘要 | Background Genome‑wide association studies (GWASs) have linked RRBP1 (ribosomal‑binding protein 1) genetic variants to atherosclerotic cardiovascular diseases and serum lipoprotein levels. However, how RRBP1 regulates blood pressure is unknown. Methods To identify genetic variants associated with blood pressure, we performed a genome‑wide linkage analysis with regional fine mapping in the Stanford Asia–Pacific Program for Hypertension and Insulin Resistance (SAPPHIRe) cohort. We further investigated the role of the RRBP1 gene using a transgenic mouse model and a human cell model. Results In the SAPPHIRe cohort, we discovered that genetic variants of the RRBP1 gene were associated with blood pressure variation, which was confirmed by other GWASs for blood pressure. Rrbp1‑ knockout (KO) mice had lower blood pressure and were more likely to die suddenly from severe hyperkalemia caused by phenotypically hypore‑ ninemic hypoaldosteronism than wild‑type controls. The survival of Rrbp1‑KO mice significantly decreased under high potassium intake due to lethal hyperkalemia‑induced arrhythmia and persistent hypoaldosteronism, which could be rescued by fludrocortisone. An immunohistochemical study revealed renin accumulation in the juxtaglomerular cells of Rrbp1‑KO mice. In the RRBP1‑knockdown Calu‑6 cells, a human renin‑producing cell line, transmission electron and confocal microscopy revealed that renin was primarily retained in the endoplasmic reticulum and was unable to efficiently target the Golgi apparatus for secretion. Conclusions RRBP1 deficiency in mice caused hyporeninemic hypoaldosteronism, resulting in lower blood pressure, severe hyperkalemia, and sudden cardiac death. In juxtaglomerular cells, deficiency of RRBP1 reduced renin intracel‑ lular trafficking from ER to Golgi apparatus. RRBP1 is a brand‑new regulator of blood pressure and potassium homeo‑ stasis discovered in this study. |
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