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頁籤選單縮合
題 名 | 應用WGS-PCR方法輔助痢疾桿菌鑑定之探討=Application of WGS-PCR Method in Assisting Identification of Shigella spp. |
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作 者 | 魏孝倫; 廖盈淑; 鄧如琇; 陳柏翰; 邱乾順; | 書刊名 | 疫情報導 |
卷 期 | 39:2 2023.01.17[民112.01.17] |
頁 次 | 頁11-18 |
分類號 | 412.42 |
關鍵詞 | 志賀氏桿菌; Shigella; WGS; PCR; |
語 文 | 中文(Chinese) |
DOI | 10.6524/EB.202301_39(2).0001 |
中文摘要 | 志賀氏桿菌會引起桿菌性痢疾,目前列為我國之第二類法定傳染病,此病原近五年每年平均造成104例的本土個案。由於志賀氏桿菌鑑定標準方法係以傳統生化試驗配合血清學分型,但此法仍無法明確鑑定部份的志賀氏桿菌,造成臨床醫學實驗室通報的困擾。為提升志賀桿菌的鑑別度,本研究以臨床分離株測試以基因體為基礎的PCR分型法(WGS-PCR),此法主要測定志賀氏桿菌基因體的各個演化枝,並搭配志賀氏菌及腸侵襲性大腸桿菌(enteroinvasive E. coli, EIEC)專一性基因IpaH的測定,來鑑定出志賀氏菌的血清群。我們以30株痢疾桿菌確認菌株進行驗證,發現WGS-PCR在血清群B、C、D的結果與血清凝集法有100%的一致性,但在血清群A的結果與血清凝集法僅有20%一致。另外以WGS-PCR測試29株疑似痢疾桿菌菌株,各有2株菌株被鑑定為血清群B及D,亦顯示WGS-PCR可用於鑑定血清群B及D菌株,可明確釐清疑似菌株。WGS-PCR只需進行低成本的PCR檢驗,我們建議可作為血清凝集法的輔助試驗,以提升志賀氏菌的鑑別度。 |
英文摘要 | Shigella spp. is the causative agent of shigellosis and one of the category 2 communicable diseases in Taiwan, and it causes an average of 104 local cases per year in recent 5 years. As traditional biochemical tests and serotyping cannot accurately identify some strains of Shigella spp., this becomes an obstacle for clinical laboratories to report shigellosis cases to the health departments. To improve the identification of Shigella spp., we tested clinical strains of Shigella spp. with the whole-genomic-based PCR (WGS-PCR) method. WGS-PCR method allows detection of both the phylogenic clades of Shigella spp. and the IpaH gene, an invasive gene plasmid of either Shigella spp. or EIEC. Among the 30 confirmed strains of Shigella spp., the results of WGS-PCR were 100% concordant with that of serotyping on group B, C and D strains, but only 20% consistency was found on group A strains. While using WGS-PCR to test 29 suspicious strains of Shigella spp., 2 group B strains and 2 group D strains were identified, indicating that WGS-PCR may clearly detect suspicious strains. As WGS-PCR method is a low-cost method, we recommend using WGS-PCR as a supplementary test for serotyping. |
本系統中英文摘要資訊取自各篇刊載內容。