頁籤選單縮合
| 題 名 | Protection of the Neurovascular Unit from Calcium-related Ischemic Injury by Linalyl Acetate |
|---|---|
| 作 者 | Hsieh, Yu Shan; Shin, You Kyoung; Seol, Geun Hee; | 書刊名 | The Chinese Journal of Physiology |
| 卷 期 | 64:2 2021.03-04[民110.03-04] |
| 頁 次 | 頁88-96 |
| 分類號 | 415.132 |
| 關鍵詞 | Calcium-related ischemic injury; Linalyl acetate; Neurovascular unit; |
| 語 文 | 英文(English) |
| 英文摘要 | Calcium-related ischemic injury (CRII) can damage cells of the neurovascular unit (NVU). Here, we investigate the protective effects of linalyl acetate (LA) against CRII-induced NVU damage and evaluate the underlying mechanisms. The protective effects of LA in cell lines representative of NVU components(BEND, SH-SY5Y, BV2, and U373 cells) were evaluated following exposure to oxygen-glucose deprivation/ reoxygenation alone (OGD/R-only) or OGD/R in the presence of 5 mM extracellular calcium ([Ca2+] o ) to mimic CRII. LA reversed damage under OGD/R-only conditions by blocking p47phox/NADPH oxidase (NOX) 2 expression, reactive oxygen species(ROS) production, nitric oxide (NO) abnormality, and lactate dehydrogenase (LDH) release only in the BEND cells. However, under CRII-mimicking conditions, LA reversed NO abnormality and matrix metalloproteinase (MMP)-9 activation in the BEND murine brain endothelial cells; inhibited p47phox expression in the human SH‑SY5Y neural‑like cells; decreased NOX2 expression and ROS generation in the BV2 murine microglial cells; and reduced p47phox expression in the U373 human astrocyte-like cells. Importantly, LA protected against impairment of the neural cells, astrocytes, and microglia, all of which are cellular components of the NVU induced by exposure to CRII-mimicking conditions, by reducing LDH release. We found that LA exerted a protective effect in the BEND cells that may differ from its protective effects in other NVU cell types, following OGD/R-induced damage in the context of elevated [Ca2+]o. |
本系統中英文摘要資訊取自各篇刊載內容。