頁籤選單縮合
題 名 | LncRNA MALAT1 Silencing Protects against Cerebral Ischemia-reperfusion Injury through miR-145 to Regulate AQP4 |
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作 者 | Wang, Hongwei; Zheng, Xiaoxiao; Jin, Jing; Zheng, Li; Guan, Ting; Huo, Yangfan; Xie, Shufen; Wu, Ying; Chen, Wei; | 書刊名 | Journal of Biomedical Science |
卷 期 | 27 2020[民109] |
頁 次 | 頁(40)1-(40)12 |
分類號 | 415.92 |
關鍵詞 | MALAT1; miR-145; Cerebral ischemia-reperfusion injury; AQP4; |
語 文 | 英文(English) |
DOI | 10.1186/s12929-020-00635-0 |
英文摘要 | Background: The present study aimed to verify whether long noncoding RNA (lncRNA) MALAT1 is involved in brain tissue damage induced by ischemia-reperfusion injury, and to explore the mechanism by which MALAT1 regulates aquaporin 4 (AQP4). Methods: In this study, we established glucose deprivation (OGD)/reoxygenation (RX) astrocyte cell model and middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vitro and in vivo. Then cell counting kit-8 assay, flow cytometry analysis, Triphenyltetrazolium chloride (TTC) staining, and western blotting were used to determine cell viability, cell apoptosis, cerebral infarction volume, and the abundance of AQP4, respectively. Results: We found that the level of MALAT1 was significantly upregulated in both the MCAO/reperfusion model and OGD/RX model. Knockdown of MALAT1 increased cell viability and reduced cell apoptosis in MA-C cells, while an AQP4 siRNA combined with a siRNA targeting MALAT1 could not enhance this effect. Further experiments showed that MALAT1 positively regulated AQP4 expression via miR-145. The MALAT1 siRNA did not alleviate the exacerbation of damage after miR-145 inhibitor action. However, an miR-145 inhibitor reversed the protection effects of MALAT1, indicating that MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145. TTC staining showed that the infracted area of whole brain was significantly attenuated in treated with sh-MALAT1 group in vivo. Conclusion: Taken together, our study confirmed that MALAT1 promotes cerebral ischemia-reperfusion injury by affecting AQP4 expression through competitively binding miR-145, indicating that MALAT1 might be a new therapeutic target for treatment cerebral ischemic stroke. |
本系統中英文摘要資訊取自各篇刊載內容。