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- 嗜氧運送培養基以較高溫度運送產前檢體可提升B群鏈球菌(Group B Streptococcus)的偵測效能
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題名 | 嗜氧運送培養基以較高溫度運送產前檢體可提升B群鏈球菌(Group B Streptococcus)的偵測效能=Transport of Prenatal Specimens with Aerobic Transport Medium at a Temperature Slightly Higher Than Room Temperature Can Enhance the Detection of Group B Streptococcus |
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作者 | 廖姮懿; 歐柏廷; 陳詩涵; 廖一凡; 蔡文城; | 書刊名 | 檢驗及品保雜誌 |
卷期 | 9:1 2020[民109] |
頁次 | 頁17-25 |
分類號 | 414.91 |
關鍵詞 | B群鏈球菌; 糞腸球菌; 嗜氧運送管; 運送時間; 運送溫度; GBS; Enterococcus faecalis; Group B Streptococcus; Aerobic transport medium; CMPᵀᴹ Aerobic Trans CultSwab; Delivery duration; Shipping temperature; |
語文 | 中文(Chinese) |
中文摘要 | B群鏈球菌Streptococcus agalactiae(Group B Streptococcus; GBS)為人類產道、直腸等常棲菌群之一,可造成新生兒肺炎(pneumonia)、腦膜炎(meningitis)和敗血症(sepsis)。前人研究指出糞腸球菌(Enterococcus faecalis)雖然較少見於泌尿生殖道系統中,但其具有抑制GBS生長的潛力(growth potential)。根據美國疾病管制局(Centers for Disease Control and Prevention,CDC)及臺灣國健署對B群鏈球菌產前檢驗檢體的採集標準、保存與運輸作業的建議。本研究以在含有糞腸球菌前提下,將1×10^3 CFU/mL的β溶血型GBS與Escherichia coli、Staphylococcus aureus、E. faecalis及Candida albicans四種菌的混合菌液分別以10:1;1:1;及1:10混合後接種至嗜氧運送管(Aerobic transport medium;CMP^(TM) Aerobic TransCultSwab)的棉拭中,並放置於不同溫度(28℃、22℃與4℃)與不同時間(6、24、48與72小時)以模擬臨床檢體的運送情境,然後接種於Carrot broth,在35℃培養隔夜後,取出棉拭移種BAP與GBS Detection Ⅱ,結果指出(i)將嗜氧運送管存放於28℃中,且GBS與其它菌之混合比例為10:1時,運送時間為6小時或24小時,對GBS有最佳的偵測效果。(ii)當GBS與E. faecalis混合菌比例為1:10時,或運送溫度為22℃或4℃時,GBS將受到E. faecalis干擾而導致不良的GBS檢出以及影響GBS Detection II上生長菌落或carrot broth的產色,但運送溫度為28℃時,仍有高GBS偵測率。綜合上述,吾等的結果顯示當將帶有接種產前檢查檢體的嗜氧運送培養基在4℃下運送至檢驗室時,無論是否存在污染細菌與無論任何運送時間,GBS的檢測率都會很差。雖然美國CDC及台灣衛福部國健署皆建議產前GBS檢查檢體應以4℃低溫方式運送,吾等的發現並不支持其等的建議。 |
英文摘要 | Streptococcus agalactiae (Group B Streptococcus; GBS) is a common colonizer of the digestive and genitourinary tracts in humans. GBS can cause diseases in neonates, such as pneumonia, meningitis, and sepsis. Although Enterococcus faecalis is not a common colonizer in the genitourinary tract, it has been shown to interfere with the growth of GBS. In this study, we assessed the effects of contaminating bacteria, specimen shipping temperature, and duration of enrichment on GBS detection. We added GBS (1×10^3 CFU/mL) to a mixture of Escherichia coli, Staphylococcus aureus, E. faecalis, and Candida albicans at 10:1, 1:1, and 1:10 ratios. Each sample was then inoculated into the aerobic transport medium (CMP^(TM) Aerobic TransCultSwab) with a swab and then store at 4, 22, or 28℃ for 6, 24, 48, or 72 h to simulate various delivery conditions. The swab was then transferred to carrot broth and incubated at 35℃ in an ambient incubator. After an overnight enrichment, the sample was inoculated on BAP and GBS Detection II and incubated overnight. Results showed the following: (i) GBS detection rate was the highest when the ratio of GBS to mixed bacteria was 10 : 1 and the sample was stored at 28℃, and (ii) When the ratio of GBS to mixed bacteria was 1:10 or when the sample was stored at 22℃ or 4℃, the growth of GBS was inhibited, but when the sample was stored at 28℃, GBS was successfully detected. Taken together, our results suggest that when an aerobic transport medium with inoculated specimen is delivered to the lab at 4℃, the detection rate of GBS will be poor, regardless of the presence or absence of contaminating bacteria and delivery duration. Although both the Centers for Disease Control and Prevention (CDC) and the Ministry of Health and Welfare (MHW) of Taiwan suggest that GBS samples be delivered at 4℃, our new findings do not support this recommendation. |
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