查詢結果分析
相關文獻
頁籤選單縮合
題名 | The Expression of Th9 and Th22 Cells in Rats with Cerebral Palsy after hUC-MSC Transplantation |
---|---|
作 者 | Niu, Xia; Xu, Xiaoyan; Luo, Zhihua; Wu, De; Tang, Jiulai; Tang, Jiulai; Luo, Zhihua; Niu, Xia; Xu, Xiaoyan; Wu, De; | 書刊名 | Journal of the Chinese Medical Association |
卷期 | 83:1 2020.01[民109.01] |
頁次 | 頁60-66 |
分類號 | 368.5 |
關鍵詞 | Cerebral palsy; hUC-MSC transplantation; Th9; Th22; |
語文 | 英文(English) |
英文摘要 | Background: This study aimed to investigate the expression of Th9 and Th22 cells in rats with cerebral palsy (CP) after human umbilical cord-derived mesenchymal stem cell (hUC-MSC) transplantation. Methods: First, hUC-MSCs were isolated from fresh umbilical cords and identified. Rats were divided into the normal group, CP group, and hUC-MSC transplantation group. The Morris water maze and balance beam tests were performed to evaluate the neurobehavioral ability of the rats. The levels of TNF-α, IL-6, IL-9, and IL-22 in rat brain tissues were detected by ELISA. Th9 and Th22 proportions in brain tissues were detected by flow cytometric analysis. The mRNA levels of IL-9, IL-22, PU.1, and AHR in brain tissues were determined by qRT-PCR. Results: hUC-MSC transplantation enhanced the neurobehavioral ability of CP rats. Furthermore, Th9 and Th22 proportions were decreased in brain tissues from CP rats after hUC-MSC transplantation. The levels of proinflammatory cytokines (TNF-α and IL-6), Th9-related IL-9 and PU.1, and Th22-related IL-22 and AHR were markedly higher in brain tissues from CP rats than in brain tissues from control rats, but their levels were significantly decreased after hUC-MSC transplantation. Conclusion: Our data indicate that Th9 and Th22 proportions are decreased in CP rats after hUC-MSC transplantation. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。