頁籤選單縮合
題名 | Cell Cycle Regulation in the Estrogen Receptor Beta (ESR2)--Overexpressing Hep3B Hepatocellular Carcinoma Cell Line |
---|---|
作 者 | Liu, Yi-sheng; Tsai, Ying-lan; Yeh, Yu-lan; Chung, Li-chin; Wen, Su-ying; Kuo, Chia-hua; Lin, Yueh-min; Vijaya Padma, V.; Bharath Kumar, V.; Huang, Chih-yang; | 書刊名 | The Chinese Journal of Physiology |
卷期 | 58:2 2015.04[民104.04] |
頁次 | 頁134-140 |
分類號 | 415.5362 |
關鍵詞 | C-myc; Cyclin D1; ESR2; Hep3B cells; |
語文 | 英文(English) |
英文摘要 | Epidemiological studies and experimental data have shown that the incidences of hepatocellular carcinoma in men are more frequent than in women. Evidence suggests that imbalance of hormones, including estrogen, androgen, prolactin, and growth hormone, modifies liver tumorigenesis. In this present study, we investigated how estrogen and estrogen receptor 2 (ESR2), regulates the cell cycle mechanism in Hep3B hepatocellular carcinoma cell line. Our results showed that ESR2 overexpression in the presence of 10-8 M 17-β-estradiol downregulated c-myc and cyclin D1 expression and simultaneously upregulated p27 expression. However, flow cytometry and MTT assays showed only minor G1 phase arrest without affecting cell viability. Taken together, these observations indicate that ESR2 is required to lower tumorigenesis in males by altering cell cycle proteins in a ligand-dependent manner. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。