查詢結果分析
來源資料
相關文獻
- 黃芹苷於皮膚角質細胞(HaCat cell)對中波紫外線之輻射保護作用
- Analysis and Comparison of Baicalin, Baicalein and Wogonin Contents in Traditional Decoctions and Commercial Extracts of Scutellariae Radix
- 搔癢症之處理
- 黃芩苷美白效能之研究
- 單次運動對間歇性低氧誘發心肌微管相關蛋白1輕鏈3-Ⅱ的影響
- 中藥黃芩及其活性成分之抗發炎作用研究
- Simultaneous Determination of Bromhexine HCI and Baicalin in Chinese Compound Medicine by a Reversed-Phase Ion-Pair HPLC
- Simultaneous Analysis of Nine Components in "Byi-Liang-Tang" Preparation by High Performance Liquid Chromatography
- 鋅在運動促進心肌保護功能降低缺血再灌流傷害的重要性
- 運動對阻塞型睡眠呼吸中止症患者心肌氧化傷害的影響
頁籤選單縮合
題名 | 黃芹苷於皮膚角質細胞(HaCat cell)對中波紫外線之輻射保護作用=Radioprotective Effects of Baicalin after UVB Exposure in HaCat Cells |
---|---|
作者 | 賴昱良; 賴韻伃; 莊曜禎; 許錦龍; 林松水; | 書刊名 | 中華放射線技術學雜誌 |
卷期 | 40:4 2016.12[民105.12] |
頁次 | 頁205-212 |
分類號 | 414.51 |
關鍵詞 | 黃芩苷; 活性氧物種; 中波紫外線; 人類皮膚角質細胞; 輻射保護作用; Baicalin; Reactive oxygen species; Ultraviolet b; HaCat cell; Radioprotective effects; |
語文 | 中文(Chinese) |
中文摘要 | 黃芩苷是類黃酮的化合物,來自中醫草藥中黃芩主要的成份,具有藥物活性、抗氧化、抗菌、抗細胞凋亡、抗發炎等特徵,研究發現在長波紫外線暴露後黃芩苷可減少過氧化物、氮氧化物及活性氧物種(reactive oxygen species)的產生。本研究旨在探討黃芩苷對於中波紫外線(UVB)暴露後於人類皮膚角 質細胞(HaCat cell)之細胞殘留分率及釐清黃芩苷對此合併處理之作用機制。黃芩苷合併處理(100 μg/ml)的細胞存活率比單獨輻射組增加27%;黃芩苷合併處理(50 μg/ml)的細胞存活率比單獨輻射組增加16%。細胞週期分析UVB 與黃芩苷(50、100 μg/ml)合併處理比較,黃芩苷合併處理與輻射單獨處理相比,其在sub-G1期比率依序減少52%、40%。在西方點墨法實驗中,加入黃芩苷處理後與單獨UVB相比可以減 少細胞蛋白酶-3與增加MCL-1之活性。結論顯示黃芩苷具有輻射保護作用,明確可以降低經由中波紫外線照射後的細胞死亡率及細胞凋亡率。 |
英文摘要 | Baicalin is a flavonid glycoside isolated from Scutellaria baicalensis. The flavones have the multiple potentials in exerting antioxidant, anti-bacterial, anti-apoptotic, anti-inflammatory. In previous studies, baicalin were reported with radioprotection mediating by decreases of reactive oxygen species (ROS), super-oxide anion (O_2 ^-), and nitogen oxide (NO) on photodamage after ultraviolet A (UVA) radiation. The aims of this study were to analyze survival fractions and to investigate the mechanisms of baicalin and ultraviolet B (UVB) in HaCat cell. In combined groups of baicalin (100, 50 μg/ml) and UVB, cell viabilities were increased 27% and 16% than UVB alone. The cells of sub-G1 stage of combined groups with baicalin of 100 and 50 μg/ml were reduced 52% and 40% than UVB alone, respectively. In the western blot, the combined groups decreased the expression of caspase-3 and increased the MCL-1 expression than UVB alone. The baicalin pretreatment has radioprotective effects, it apparently reduces cell death and apoptosis rate in the human keratinocyte cell after UVB exposure. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。