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題名 | 本土綠殭菌對大鼠急毒性與致病性之安全評估=Safety Evaluation of Acute Toxicity/Pathogenicity of Nomuraea Rileyi F055 in Rats |
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作者 | 張敬宜; 黃振聲; 蔡三福; Chang, J. Y.; Hwang, J. S.; Tsai, S. F.; |
期刊 | 植物保護學會會刊 |
出版日期 | 20061200 |
卷期 | 48:4 民95.12 |
頁次 | 頁331-340 |
分類號 | 433.85 |
語文 | chi |
關鍵詞 | 綠殭菌; 口服毒性; 肺毒性; 大鼠; Nomuraea rileyi; Oral toxicity; Pulmonary toxicity; Rat; |
中文摘要 | 以強迫投予方式檢測綠殭菌 (Nomuraea rileyi) 孢子對大鼠口服及肺急毒性與致病性安全評估,分別以胃管及氣管針投予1x108 cfu孢子,觀察21天內孢子分佈及清除之情形,評估其在大鼠體內之存活及對肺臟之影響。試驗結果顯示在口服急毒性與致病性方面,大鼠無任何明顯之臨床症狀或死亡現象;試驗組大鼠之體重增加與對照組無明顯之差異;所有供試鼠隻進行大體解剖及肉眼觀察無明顯病變;處理後第1天進行消化道、糞材、各臟器及血液微生物之培養,未發現綠殭菌菌落。進一步探討孢子的消化穩定性,以觀察孢子被人工胃腸液降解情形,發現在37℃胃液反應2小時後,1x 108 cfu活孢子均被完全降解而不具活性,因此,顯示綠殭菌孢子對大鼠不具口服急毒性、感染性及致病性。在肺急毒性與致病性方面,以氣管針灌注投予後,處理組大鼠均產生明顯的呼吸音(水泡音)及體重增重減少之臨床症狀,但無致死性;微生物培養只在投予後第1天的肺臟組織中發現綠殭菌菌落。肉眼觀察可見處理組肺臟潮紅腫脹,切片可見多發性肉芽腫病變,唯此病灶屬於正常的異物性肺炎反應。又以 Periodic Acid-Schiff's (PAS) 特殊染色法檢測綠殭菌孢子在肺臟組織的發育情形,發現處理組的肺臟組織中含多量被染成紫紅色的綠殭菌孢子,且孢子的形態完整,可見孢子未在大鼠的肺臟組織中發育,因此,綠殭菌孢子對大鼠不具肺急毒性、感染性及致病性。本試驗顯示綠殭菌孢子對大鼠無明顯的口服及肺急毒性與致病性,且孢子易在消化道被降解而無法存活。此試驗研究資料,可提供對本綠殭菌菌株開發產品安全性毒理資料之參考。 |
英文摘要 | This study was conducted to evaluate the acute oral and pulmonary toxicity/pathogenicity of Nomuraea rileyi after a single oral or intratracheal administration of 1x108 spores in rats. The distribution and clearance of spores in rats were observed for 21 days. Animals with oral administration showed no significantly toxic signs or death, and no significant differences in body weight gain and gross or microscopic pathology compared to control rats. Fungal spores were not detected in gut content, feces, organ tissues or blood within one day after administration. When digestive stabilities of spores in a simulated gastric fluid or a simulated intestinal fluid were tested, 1x108 spores added were completely degraded within 2 hours in simulated gastric fluid at 37℃. These data demonstrated that N. rileyi showed no obvious evidence of oral toxicity or pathogenicity/infectivity in rats. In the acute pulmonary toxicity/pathogenicity study, rats with clinical signs showed respiration-rales in all treatment groups. The body weight gain decreased significantly in the treated rats compared with control groups. The spores were found in lungs on the 1st day, but not in other organs or blood during test periods. Gross findings revealed that the lungs of treated rats showed the sign of focal pneumonitis. Histopathological lesions characterized by the inflammatory changes were observed within both bronchioles and alveoli, and foamy cells were subsequently formed in the lungs. There was no sign of spore germination in tissues after administration. These data demonstrated that N. rileyi showed no pulmonary toxicity or pathogenicity/infectivity in rats. The above results confirm the safety of N. rileyi for rats, and show that spores would be degraded in the intestinal tracts. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。