頁籤選單縮合
題名 | Polymerase Chain Reaction Assay for the Detection of Acinetobacter baumannii in Endotracheal Aspirates from Patients in the Intensive Care Unit= |
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作者 | Chiang, Mei-chun; Kuo, Shu-chen; Chen, Yu-chih; Lee, Yi-tzu; Chen, Te-li; Fung, Chang-phone; |
期刊 | Journal of Microbiology, Immunology and Infection |
出版日期 | 20110400 |
卷期 | 44:2 2011.04[民100.04] |
頁次 | 頁106-110 |
分類號 | 415.15 |
語文 | eng |
關鍵詞 | Acinetobacter baumannii; Detection; Diagnosis; Polymerase chain reaction; PCR; |
英文摘要 | Background: We aim to evaluate the efficacy of polymerase chain reaction (PCR) to detect Acinetobacter baumannii in endotracheal aspirates. Methods: Endotracheal aspirates and clinical data were collected from patients who were admitted to the intensive care unit of Taipei Veterans General Hospital between April 1 and August 31 in 2006. Bacterial isolates from endotracheal aspirate cultures were phenotypically identified as Acinetobacter calcoaceticuseA baumannii complex using the API ID 32GN system. The presence of A baumannii in the aspirate was also directly detected by multiplex PCR. Results: Ten of the 114 endotracheal aspirate cultures were positive for A calcoaceticuse A baumannii complex, and only nine of the isolates were confirmed as A baumannii by the multiplex PCR. Direct PCR detection showed that 40 (35.1%) of the endotracheal aspirates were positive for A baumannii. Using positive culture of A baumannii as the gold standard, the sensitivity of direct PCR detection was 100% (6 of 6), the specificity was 70.4% (38 of 54), the positive predictive value was 27.3% (6 of 22), and the negative predictive value (NPV) was 100% (38 of 38) among patients with A baumannii pneumonia. Among patients with A baumannii colonization, the sensitivity of direct PCR detection was 100% (3 of 3), the specificity was 70.6% (36 of 51), the positive predictive value was 16.7% (3 of 18), and the NPV was 100% (36 of 36). Conclusion: Direct PCR detection of A baumannii in endotracheal aspirates has a high sensitivity and NPV as compared with culture-based methods. Further studies are needed to determine the clinical applicability of this rapid detection test. |
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