頁籤選單縮合
題 名 | Emergence and Dissemination of blaOXA-23-carrying Imipenem-resistant Acinetobacter sp in a Regional Hospital in Taiwan |
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作 者 | Lin, Ming-feng; Kuo, Han-yueh; Yeh, Hui-wen; Yang, Chih-man; Sung, Chih-hung; Tu, Chi-chao; Huang, Mei-luan; Liou, Ming-li; | 書刊名 | Journal of Microbiology, Immunology and Infection |
卷 期 | 44:1 2011.02[民100.02] |
頁 次 | 頁39-44 |
分類號 | 419.38 |
關鍵詞 | Acinetobacter sp; blaOXA-23 gene; blaOXA-51 gene; ISAba1; Pulsed-field gel electrophoresis; |
語 文 | 英文(English) |
英文摘要 | Background: The distribution and characterization of OXA-type carbapenemases in Acinetobacter sp in Taiwan has less been reported. The aim of the study was to investigate the molecular epidemiology and OXA-type carbapenemase genes in a regional hospital in Taiwan. Methods: Imipenem-resistant Acinetobacter sp were collected between 2005 and 2007 in a regional hospital. Genotyping was performed by pulsed-field gel electrophoresis. OXA-type carbapenemase genes were determined by multiplex polymerase chain reaction (PCR) and gene sequencing. Results: A total of 136 isolates were collected. Fifty-six pulsotypes were identified. None of the pulsotypes established predominance throughout the 3-year period. Multiplex PCR of blaOXA genes showed that 99% (135/136) of the Acinetobacter sp possessed blaOXA51-like genes. The coexistences of blaOXA51-like/blaOXA-23-like and blaOXA51-like/blaOXA-24-like were detected in 19% (26/136) and 1% (2/136) of the isolates, respectively. Among blaOXA-23-like gene-carrying isolates, two isolates (Pulsotypes 18 and 20) were found in 2006 and the remainder (n Z 24), including Pulsotypes 27 (n Z 18), 29 (n Z 1), 52 (n Z 3), and 53 (nZ2), were found in 2007. Sequencing performed on the 26 representative isolates confirmed the presence of the blaOXA-23 carbapenemase gene. Analysis of the genetic content of blaOXA-23 showed that these genes were presumably chromosomal and associated with the upstream-located insertion sequence ISAba1. Conclusions: The emergence and imminent widespread of blaOXA-23-carrying imipenemresistant Acinetobacter sp appeared in Taiwan during the period from 2006 to 2007. |
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