頁籤選單縮合
題名 | Recombinant Tuberculosis Vaccine AEC/BC02 Induces Antigen-specific Cellular Responses in Mice and Protects Guinea Pigs in a Model of Latent Infection |
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作者姓名(外文) | Lu, Jin-biao; Chen, Bao-wen; Wang, Guo-zhi; Fu, Li-li; Shen, Xiao-bin; Su, Cheng; Du, Wei-xin; Yang, Lei; Xu, Miao; | 書刊名 | Journal of Microbiology, Immunology and Infection |
卷期 | 48:6 2015.12[民104.12] |
頁次 | 頁597-603 |
分類號 | 414.82 |
關鍵詞 | CpG adjuvant; Guinea pig; Latent tuberculosis infection; Mycobacterium tuberculosis; Recombinant vaccine; |
語文 | 英文(English) |
英文摘要 | Purpose: To preliminarily evaluate the immunogenicity and efficacy of the recombinant tuberculosis vaccine AEC/BC02 in which Ag85b and fusion protein ESAT6-CFP10 were combined with bacillus Calmette-Gue´rin CpG and an aluminum salt-based adjuvant system. Methods: Groups of BALB/c mice were immunized intramuscularly three times at 10-day intervals with AEC/BC02 or the adjuvant alone and the vaccine-induced cell-mediated immune responses were evaluated. The efficacy of AEC/BC02 was evaluated in two guinea pig models, one a model of prevention and the other a model of latent infection. Results: The AEC/BC02 vaccine induced strong cellular immune responses characterized by a high frequency of antigen-specific interferon-g-secreting T cells in mice at different time points after the last vaccination. In the preventive model of guinea pig, AEC/BC02 did not protect against Mycobacterium tuberculosis as a pre-exposure vaccine. However, in a latent infection model of guinea pig, it effectively controlled the reactivation of M. tuberculosis and lowered the bacterial load in the lung and spleen. Conclusion: These results indicate AEC/BC02 can protect against reactivation of latent infection and may function as a therapeutic vaccine. |
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